. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NEUROBIOLOGY 140 120 Oxygen 400(cm -.' !i 200|im f^ 800|im 1 100 100nm v . 80 1 ^— f* 60 J-* 0 100 200 300 400 500 A Time (sec) -160 V P -170 £ -180 -190 -200 B ' T V ' >*« _ | 800Mm 100(im ^"*««> 1 1 ) 50 100 150 200 250 30 Time (sec). 250 200 » a wave _ ^ 150 • b wave • • A d wave • ..^ 100 .A 50 A \iV o Q -50 » »» »»»» -100 Log Relative Intensity D Figure 1. Measurements of oxygen. pH. and the electroretinogramfrom the everted turtle eyectip preparation. (A) Response from an oxygen-se
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NEUROBIOLOGY 140 120 Oxygen 400(cm -.' !i 200|im f^ 800|im 1 100 100nm v . 80 1 ^— f* 60 J-* 0 100 200 300 400 500 A Time (sec) -160 V P -170 £ -180 -190 -200 B ' T V ' >*« _ | 800Mm 100(im ^"*««> 1 1 ) 50 100 150 200 250 30 Time (sec). 250 200 » a wave _ ^ 150 • b wave • • A d wave • ..^ 100 .A 50 A \iV o Q -50 » »» »»»» -100 Log Relative Intensity D Figure 1. Measurements of oxygen. pH. and the electroretinogramfrom the everted turtle eyectip preparation. (A) Response from an oxygen-selective microelectrode as it was withdrawn with time in a stepwise manner from the surface of the retina. The first four steps were 50 fjun each: thereafter, the electrode was moved in 100-fim steps until the last two. which were 200 yjn. For ease of interpretation, arrows indicate the distances from the retina at . 2, 4. 6. and 10. (B) pH gradient measured from a second eyecup as a function of distance from the retinal surface. The first four steps were 50 /nm, the next four 100 and the last Mo 200 Arrows indicate the distances from the retina at steps 2, 4, 6, and 10. (C) Representative ERG responses that were elicited by light stimuli of three different intensities (log relative intensity of and ). Calibration: vertical scale. 50 fjiV. horizontal scale, 100 ms. The upper trace indicates stimulus timing. {D} Intensity-response cun'esfor the ERG a-mive, h-\vave, and d-wave. The amplitudes of these waves in are plotted as a function of log relative intensity. used as a model preparation for examining retinal function (2. 3. 4). Here we have developed a new variation of the turtle eyecup preparation, which permits us to examine metabolic parameters, including oxygen consumption and changes in extracellular hy- drogen ion concentration, while simultaneously assessing the physiological state of retinal function from the e
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
