. Contributions from the Botanical Laboratory, vol. 7. Botany; Botany. When cells of the monocyte or fibroblast type which have abundant fat are fixed and stained by the usual osmotic acid and iron haemato- xylin method, the former fat globules now appear as (white) vacuoles (Fig. 10, PI. II; Fig. 6). Fell and Andrews (14) and D. H. Strangeways (in as yet unpublished work) have described hypertrophied cells in old (8 day) cultures (of Jensen's rat sarcoma in blood serum) which have a central mass of fat globules (Fig. 6, A). On close examination these globules are seen to be partially flattene


. Contributions from the Botanical Laboratory, vol. 7. Botany; Botany. When cells of the monocyte or fibroblast type which have abundant fat are fixed and stained by the usual osmotic acid and iron haemato- xylin method, the former fat globules now appear as (white) vacuoles (Fig. 10, PI. II; Fig. 6). Fell and Andrews (14) and D. H. Strangeways (in as yet unpublished work) have described hypertrophied cells in old (8 day) cultures (of Jensen's rat sarcoma in blood serum) which have a central mass of fat globules (Fig. 6, A). On close examination these globules are seen to be partially flattened tending toward the hexagon in cross section (Fig. 6, B). On fixation, the now empty globules (fat "vacuoles'") show a very pronounced hexagonal shape giving a distinctly alveolar appearance to the cytoplasmic skeleton (Fig. 6, C). The central area of fat droplets stops sharply where the broad hyaline zone begins in these giant cells, a condition also found in Euplotes as already referred to. This fact would not attract attention were the emulsion a loose one, but here the globules are under sufficient pressure to cause partial deformation, yet they do not spread into the peripheral zone. Fig. 5. Drawing of one of the tissue-culture (chick heart) cells pictured in the photomicrographs Fig. 8, PL II (taken with dark-field) and Fig. 9, PI. II (taken with direct light), showing here the distribution of U^ Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original University of Pennsylvania. Botanical Laboratory. Philadelphia : University of Pennsylvania Press


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Keywords: ., bookcentury1800, bookdecade1890, booksubjectbotany, bookyear1892