. Journal of applied microscopy and laboratory methods. uch useof them. Of late, carbon-dioxide has been much utilized, especially by patholo-gists, as a means of freezing tissues for sectioning. The convenience with which and Laboratory Methods. 1321 fluid carbon-dioxide may be obtained in tanks, and its power of rapid freezing,have caused it to be preferred to ether and similar fluids. In every active patho-logical laboratory the freezing microtome is in daily use. Perhaps its greatest valuelies in the fact that thin sections may be made within a few minutes after theremoval of tissue from t
. Journal of applied microscopy and laboratory methods. uch useof them. Of late, carbon-dioxide has been much utilized, especially by patholo-gists, as a means of freezing tissues for sectioning. The convenience with which and Laboratory Methods. 1321 fluid carbon-dioxide may be obtained in tanks, and its power of rapid freezing,have caused it to be preferred to ether and similar fluids. In every active patho-logical laboratory the freezing microtome is in daily use. Perhaps its greatest valuelies in the fact that thin sections may be made within a few minutes after theremoval of tissue from the body, and in a few minutes more these sections maybe hardened, stained, cleared, and mounted. The surgeon may thus be given apositive diagnosis of the microscopic condition of diseased tissues while heproceeds with an operation. The carbon-dioxide microtomes commonly used have, however, several draw-backs which have served to render them far less useful than they should the practical standpoint their most serious drawbacks are a tendency to. Fig. I. A. Cover of freezing stage ; B. Glass track for carrying knife ; E. Spiral spring ; F. Tubal base of knife-stage ; I. Wheel : J. Nut for attaching axial tube to tank; M. Handle of tank-valve; N. Pointer. become clogged and a great wastefulness of gas. From the scientific standpoint,lack of control over the temperature of the freezing stage serves to give rise toan over-freezing, which produces marked alterations in the tissues. In orderto remedy these defects the machine described below was devised. In designinga practical machine I had the able assistance of Mr. E. F. Northrup. TheBausch & Lomb Company, who have undertaken its manufacture, have alsooffered suggestions that have proved of much value. Figure 1 shows the machine as it stands ready for use. It is supporteddirectly by the nozzle of the carbon-dioxide tank. This offers a firm and con-venient means of attachment, but, if desired, a heavy tubing may be utilized
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Keywords: ., bookcentury1900, bookdecade1900, booksubjectmicrosc, bookyear1901
