. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 62 M. CONRAD ET AL. Figure 6. Time-course of microtuhule redistribution during clot formation by lipopolysaccharide-activated amebocytes, observed by anti-tubulin immunofluorescence (phase contrast-fluorescence pairs: time of fixation is in upper left corner of phase images a-d). (a, a') Cells 30-s post-activation; MBs are still at the periphery, somewhat folded and buckled, but still recognizable ( a', arrow); most granules have already exocytosed, and boundaries of individual cells are beginning to be obscured by the
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 62 M. CONRAD ET AL. Figure 6. Time-course of microtuhule redistribution during clot formation by lipopolysaccharide-activated amebocytes, observed by anti-tubulin immunofluorescence (phase contrast-fluorescence pairs: time of fixation is in upper left corner of phase images a-d). (a, a') Cells 30-s post-activation; MBs are still at the periphery, somewhat folded and buckled, but still recognizable ( a', arrow); most granules have already exocytosed, and boundaries of individual cells are beginning to be obscured by the clot as viewed in phase contrast (a), ib. b') 3 min after activation; microtubules have moved into the interior and are associated with nuclei; many MBs are still recognizable as highly twisted forms (, arrow), (c. c') 7 min post-activation; MBs are essentially unrecognizable as such, but microtubule bundles are still localized at nuclear surfaces (n; arrow in c'). (d, d'l 10 min post-activation; microtuhules have a much more diffuse distribution, even in the few remaining cells in which exocytosis is incomplete (d, arrow). Bar = 10 | Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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