. The eggs of mammals. Ovum; Embryology -- Mammals; Mammals -- physiology; Ovum. FERTILIZATION AND CLEAVAGE 81 actual penetration and pronucleus formation occurred in his attempts to fertilize rabbit eggs with rat sperm. No figures showing actual sperm penetration are given in this paper. The nuclear configurations shown may, in fact, occur in. Fig. 26. Rabbit ovum from sterile mat- ing treated with trypsin solution. Note many "polar" bodies. See text. ova cultured in vitro with no sperm added (see Chapter VIII). The inamediate effect of semination (and fertilization) upon mammalian
. The eggs of mammals. Ovum; Embryology -- Mammals; Mammals -- physiology; Ovum. FERTILIZATION AND CLEAVAGE 81 actual penetration and pronucleus formation occurred in his attempts to fertilize rabbit eggs with rat sperm. No figures showing actual sperm penetration are given in this paper. The nuclear configurations shown may, in fact, occur in. Fig. 26. Rabbit ovum from sterile mat- ing treated with trypsin solution. Note many "polar" bodies. See text. ova cultured in vitro with no sperm added (see Chapter VIII). The inamediate effect of semination (and fertilization) upon mammalian ova is a definite shrinkage of the vitellus (Pincus and Enzmann, 1932). Quantitative estimates of this shrinkage in rat eggs have been made by Gilchrist and Pincus (1932). In Table XII are presented their data on folhcular and tubal ova. They show that a 14 per cent reduc- tion in volume occurs in fertilized tubal ova. Furthermore, when unfertilized ova are exposed to sperm suspensions a similar shrinkage occurs (Table XIII). This shrink- age is not due to polar body extrusion since it occurs in vitro within 5 to 10 minutes, and polar bodies are normally ex- truded at 45 minutes to 1 hour after semination in vitro (Long, 1912). The ova apparently increase somewhat in volume after this initial shrinkage. Krasovskaja (1935a). Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Pincus, Gregory, 1903-1967. New York, The Macmillan Company
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