. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. COMPARATIVE BIOCHEMISTRY AND TOXICOLOGY 249. Figure 1. Effects of N-monomethyl-L-arginine on hydrogen peroxide formation during the fertilization of sea urchin eggs. Eggs, immobilized on poly-L-lysine coated tissue culture plates, were incubated for 30 nun with DCFH-DA (5 nM), incubated with sperm, and. after 10 min. analyzed with a Meridian ACAS anchored cell analysis system. Representative images are presented. Panel A. untreated fertilized eggs: panel B. eggs treated with I mM L-arginine: panel C. eggs treated with 1 mM N
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. COMPARATIVE BIOCHEMISTRY AND TOXICOLOGY 249. Figure 1. Effects of N-monomethyl-L-arginine on hydrogen peroxide formation during the fertilization of sea urchin eggs. Eggs, immobilized on poly-L-lysine coated tissue culture plates, were incubated for 30 nun with DCFH-DA (5 nM), incubated with sperm, and. after 10 min. analyzed with a Meridian ACAS anchored cell analysis system. Representative images are presented. Panel A. untreated fertilized eggs: panel B. eggs treated with I mM L-arginine: panel C. eggs treated with 1 mM NMMA; panel D. eggs treated with 1 mM NMMA in the presence of I mAf L-argininc. The color bar represents increasing fluorescence on a four- decade log scale. Each panel . a representative field from 12 different scans. Reference: Biol. Bull 187: 249-250. (October, 1994) Enzymatic Hydrolysis of Tetriso as a Model for the Detoxication of the Neurotoxic Agent VX Francis C. G. Hoskin (Marine Biological Laboratory) and John E. Walker The enzymatic hydrolysis and thus detoxication of certain organophosphorus acid anhydrides, commonly termed "nerve gases," is now well documented (1). Some of these are P-F com- pounds; all are powerful acetylcholinesterase (AChE) inhibitors, which is the basis of their neurotoxicity. One such compound, Soman, is hydrolyzed by a particular organophosphorus acid anhydrolase (OPAA) found only in certain tissues of the ceph- alopods. notably squid nerve, from which the enzyme has been purified (1). A second generation of these highly toxic compounds is typified by the military agent VX (2). Now, with a decline in international confrontation, mild means of destruction are being sought for all of these agents (3). Although VX is both extremely hazardous and generally not available, a close analog, Tetriso. originally synthesized for use in neurophysiological experiments (4). is now revealing for the first time a detoxication pathway directly a
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