. Bacteriophages. Bacteriophages; Bacteriophages. STAGES IN PHAGE MULTIPLICATION 179 out by Luria and Latarjet (1947) and by Benzer (1952). The results with this phage are strikingly different from those ob- tained with T7. Immediately after infection, the infective cen- ters have the same sensitivity to ultraviolet inactivation as free phage, but there follows an increase in resistance to inactivation until, at 5 or 6 minutes after infection, the resistance has 100 200 300 UV DOSE IN SECONDS Figure 6. Survival of phage-producing capacity of bacteria infected with T7. The bacteria we
. Bacteriophages. Bacteriophages; Bacteriophages. STAGES IN PHAGE MULTIPLICATION 179 out by Luria and Latarjet (1947) and by Benzer (1952). The results with this phage are strikingly different from those ob- tained with T7. Immediately after infection, the infective cen- ters have the same sensitivity to ultraviolet inactivation as free phage, but there follows an increase in resistance to inactivation until, at 5 or 6 minutes after infection, the resistance has 100 200 300 UV DOSE IN SECONDS Figure 6. Survival of phage-producing capacity of bacteria infected with T7. The bacteria were irradiated at times after infection indicated on the curves in minutes. Latent period (at 37° C.) 12 minutes. Reproduced from S. Benzer, 1952, J. BacterioL, 63, 59, with permission. Thereafter the resistance increases with great rapidity until maximum resistance, 20-fold greater than that of free phage, is reached at about 10 minutes after infection. At this time the in- activation curves become definitely multiple hit in character. From 10 minutes to the end of the latent period the multiplicity of the inactivation curves increases while the slope of the curve. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Adams, Mark Hancock, 1912-1956. New York, Interscience Publishers
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