. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . if absolute anaerobiasis is requiredpyrogallic acid may be used to absorb this last trace as above, allowing thewater to be sucked back before the negative pressure is completely relieved bythe hydrogen. In relieving the negative pressure it may be necessary to use apneumatic trough to collect the hydrogen, as the generation of hydrogen wiU betoo slow and air will be drawn through the Kipp apparatus. It is advisablenot to relieve the negative pressure completely, as this helps to hold the lidtightl
. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . if absolute anaerobiasis is requiredpyrogallic acid may be used to absorb this last trace as above, allowing thewater to be sucked back before the negative pressure is completely relieved bythe hydrogen. In relieving the negative pressure it may be necessary to use apneumatic trough to collect the hydrogen, as the generation of hydrogen wiU betoo slow and air will be drawn through the Kipp apparatus. It is advisablenot to relieve the negative pressure completely, as this helps to hold the lidtightly in place, and furthermore, the subsequent expansion when the jar isincubated will tend to loosen the lid and allow inward diffusion of air. Whenthe pyrogaUic method is used, the tubes or plates must be raised above thelevel of the fluid. Of the methods given, the second and third are bhe mostsatisfactory. The absorption method may be used alone, either using a jarto hold the tubes or plates, or individual plates or tubes may be handled asin the following: CULTIVATION OF MICROORGANISMS 121.
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