Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . coagulum(reserve the filtrate) and test it as follows: (a) Millons Reaction.—Make the test according to direc-tions given on page 44. (b) Xanthoproteic Test.—Make the test according to di-rections given on page 44. 4. Sugar in Serum.—Test a little of the filtrate from Ex-periment 3 by Fehlings test. What do you conclude ? 5. Detection of Sodium Chloride.— (a) Test a little ofthe filtrate from Experiment 3 for chlorides, by the use ofnitric acid and
Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . coagulum(reserve the filtrate) and test it as follows: (a) Millons Reaction.—Make the test according to direc-tions given on page 44. (b) Xanthoproteic Test.—Make the test according to di-rections given on page 44. 4. Sugar in Serum.—Test a little of the filtrate from Ex-periment 3 by Fehlings test. What do you conclude ? 5. Detection of Sodium Chloride.— (a) Test a little ofthe filtrate from Experiment 3 for chlorides, by the use ofnitric acid and argentic nitrate, (b) Evaporate 5 of thefiltrate from Experiment 3 in a watch glass on a the crystals and compare them with those reproducedin Fig. 60, page 167. 6. Separation of Serum Globulin and Serum Albunin.—Place 10 of blood serum in a small beaker and saturatewith magnesium sulphate. What is this precipitate? Filterit off and acidify the filtrate slightly with acetic acid. Whatis this second precipitate? Filter this precipitate off and testthe filtrate by the biuret test. What do you conclude ?. 167 £#30 Sodium Blood Plasma. i. Preparation of Oxalated Plasma.—Allow arterialblood to run into an equal volume of per cent ammoniumoxalate solution. 2. Preparation of Fibrinogen.—To 25 of oxalatedplasma add an equal volume of saturated sodium chloride solu-tion. Note the precipitation of fibrinogen. Filter off the pre-cipitate (reserve the filtrate) and test it by a proteid color testI see page 44). 3. Effect of Calcium.—Place a small amount of oxalatedplasma in a test-tube and add a few drops of a 2 per centcalcium chloride solution. What occurs? Explain it. 4. Preparation of Salted Plasma.—Allow arterial bloodto run into an equal volume of a saturated solution of sodiumsulphate or a 10 per cent solution of sodium chloride. Keepthe mixture in a cold place for about twenty-four hours. 5. Effect of Dilution.
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