. Urinary analysis and diagnosis by microscopical and chemical examination. alcohol and in water, andmay either be examined at once or, better, are recolored with methyleneblue, again rinsed in water, and examined in water or Canada tubercle bacilli, if any are present, will now be seen in the form ofred rods, while all other features in the specimen are colored blue. In-stead of aniline fuchsin, aniline gentian violet can be used, and vesuvin MICBO-ORGANISMS AND ANIMAL PABASITW. 157 employed as a recoloring agent. Muriatic or sulphuric acid may be usedinstead of nitric acid. A spec
. Urinary analysis and diagnosis by microscopical and chemical examination. alcohol and in water, andmay either be examined at once or, better, are recolored with methyleneblue, again rinsed in water, and examined in water or Canada tubercle bacilli, if any are present, will now be seen in the form ofred rods, while all other features in the specimen are colored blue. In-stead of aniline fuchsin, aniline gentian violet can be used, and vesuvin MICBO-ORGANISMS AND ANIMAL PABASITW. 157 employed as a recoloring agent. Muriatic or sulphuric acid may be usedinstead of nitric acid. A specimen of tuberculosis of the kidney, colored in this manner, isshown in Fig. 80. The features which can easily be recognized are tuber-cle bacilli in moderate numbers, pus-corpuscles, epithelia from the convo-luted tubules of the kidney, epithelia from the pelvis of the kidney, mu-cus-threads, mucus-corpuscles, and various cocci. Although specimens prepared with an aniline water solution give ex-cellent results, there is one objection to this method, which is that the so-. Fig. 80.—Tuberculosis of the Kidney (X 650).TB, Tubercle bacilli; PC, pus-corpuscle; CE, epithelium from convoluted tubules of kidney; PE, epithelium from pelvis of kidney:puscle. MS, mucus-threads; MC, mucus-cor- lution does not keep, and has to be prepared fresh every week. Manybacteriologists, therefore, prefer to use Ziehl-Neelsens carbolic acid fuch-sin method. The solution is prepared by taking 90 parts of a five percent carbolic-acid solution, 10 parts of alcohol, and 1 part of fuchsin insubstance. This is undoubtedly the simpler method, and gives good re-sults as long as the solution is not too old. With it the specimens neednot be colored longer than one or two hours when kept at the temperatureof the room, or ten minutes when dropped in a warm solution. The de-colorizing and recoloring processes are exactly the same as with theKoch-Ehrlich-Weigert method. Since examination for tubercle bacilli in u
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