. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Miscellaneous Dyes 231 Fl5 TITAN YELLOW G C. I. NO. 813 Synonym: Thiazol yellow. S SOiNa SOiNa S I C. C28Hi9N506S4Na2; Mol. Wt. [An acid dye) This dye has been recommended as an indicator for determining magnesium in body fluids. After removing the organic matter and calcium from such solutions the dye is added; under alkaline conditions it becomes red in the presence of magnesium and the color observed can be compared wi
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Miscellaneous Dyes 231 Fl5 TITAN YELLOW G C. I. NO. 813 Synonym: Thiazol yellow. S SOiNa SOiNa S I C. C28Hi9N506S4Na2; Mol. Wt. [An acid dye) This dye has been recommended as an indicator for determining magnesium in body fluids. After removing the organic matter and calcium from such solutions the dye is added; under alkaline conditions it becomes red in the presence of magnesium and the color observed can be compared with that of a standard previously prepared. Also, like the two preceding dyes, it has been employed in fluores- cence microscopy; it is one of those recommended by Pick (1935) and by Jenkins (1937) as a vital stain under ultra-violet illumina- tion. Fl7 THIOFLAVINE T C. I. NO. 815 Synonym: Thioflavine T G CH3_/\_S \ /~\_N_CH3 _ N=C-\_/ \ 1\ CH3 CI CH3 Ci7 Hi9 No SCI; Mol. Wt. {A basic dye) Like the other related dyes here described, this one has proper- ties as a fluorochrome; thus Metcalf and Patton (1944) have em- ployed it, with ultraviolet light, in insect histology. f20 THIOFLAVINE S C. I. NO. 8l6 {An add dye; a methylated and sulfonated derivative of primulin) This is among the dyes that has recently come into use in con- nection with fluorescence microscopy. Pick (1935) employed a thioflavine as a vital stain, depending on its fluorescence under ultra-violet light; probably he referred to this although there is also a thioflavine T (C. I. No. 815). More recent authors (Jenkins, 1937, Levaditti, et al., 1940) definitely refer to thioflavine S, and. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975. Geneva, N. Y. , Biotech Publications
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