. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 5-HYDROXYTRYPTAMINE IN P. CLARKII 343. 02468 10 02468 10 MINUTES Figure 1. Chromatograms of (A) brain supernatant of Praca mbarus clarkii and (B) aqueous standard containing 5-HT and DHBA. Column: 5 ^m silica C-18 steel. Mobile phase: methane sulphonic acid and phos- phoric acid in methanol. Detector: glassy carbon electrode at + V potential. Retention time for 5-HT min and for DHBA min. of Hogben and Slome (1931) wherein stage 1 indicates maximal pigment concentration, stage 5 maximal pig- ment dispersion, a
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 5-HYDROXYTRYPTAMINE IN P. CLARKII 343. 02468 10 02468 10 MINUTES Figure 1. Chromatograms of (A) brain supernatant of Praca mbarus clarkii and (B) aqueous standard containing 5-HT and DHBA. Column: 5 ^m silica C-18 steel. Mobile phase: methane sulphonic acid and phos- phoric acid in methanol. Detector: glassy carbon electrode at + V potential. Retention time for 5-HT min and for DHBA min. of Hogben and Slome (1931) wherein stage 1 indicates maximal pigment concentration, stage 5 maximal pig- ment dispersion, and stages 2, 3, and 4 the intermediate conditions. The Hogben and Slome stages for the exper- imental and control animals were then used to calculate Standard Integrated Responses (SIR) of the erythrophores of Uca pugilator to the nervous tissue extracts ofProcam- barus clarkii according to the method of Fingerman el al. (1967). Briefly, when pigment dispersion occurs, the sum of the Hogben and Slome stages recorded for the duration of the experiment for the control group is sub- stracted from the corresponding sum for the experimental group. The difference is the SIR. The SIR integrates the amplitude, which is based on the observed Hogben and Slome stages, and duration of the response of the ery- throphores. A dose of 50 n\ containing the tissue extract equivalent to either one eyestalk. brain, subesophageal, or thoracic ganglion was injected into each crab. For experiments involving in vitro electrical stimula- tion, the entire optic tract, including the major ganglia and sinus gland, was removed from the eyestalk and maintained in 50 ^1 physiological saline. The remaining nervous tissue from the brain to the end of thoracic nerve cord was also dissected out intact and carefully placed in 100 /jl of physiological saline. Electrical stimulation of the isolated tissue was performed as described in detail by Quackenbush and Fingerman (1984). Briefly, the eye- stalk tissue was he
Size: 1767px × 1415px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
