. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ALGAL PATHOGEN IN A GORGONIAN CORAL 369. FIGURE 1. Pseudoplexauraflagellosa (Houttuyn) with algal nodules, depth 3 m, Bache Shoal, Florida Keys. MATERIALS AND METHODS Isolation and culture of the alga Tumors were collected from several species of Pseudoplexaura in Florida and the Netherlands Antilles. Algal filaments were removed by forceps from surface sterilized (alcohol) tumors and washed repeatedly on Whatman filters with artificial sea water (Baumann et al, 1971). Sterile technique was used in all procedures. The alga w
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ALGAL PATHOGEN IN A GORGONIAN CORAL 369. FIGURE 1. Pseudoplexauraflagellosa (Houttuyn) with algal nodules, depth 3 m, Bache Shoal, Florida Keys. MATERIALS AND METHODS Isolation and culture of the alga Tumors were collected from several species of Pseudoplexaura in Florida and the Netherlands Antilles. Algal filaments were removed by forceps from surface sterilized (alcohol) tumors and washed repeatedly on Whatman filters with artificial sea water (Baumann et al, 1971). Sterile technique was used in all procedures. The alga was isolated in pure culture by streaking agar plates containing Provasoli enrichments (McLachlan, 1973) added to artificial or natural sea water with the addition of 10 mM Tris buffer, pH Cultures were grown at 25°C under a bank of cool white fluorescent lamps at 1000 lux on a 12:12 cycle. Within two weeks, dark green colonies (see Results, Fig. 4) became visible on the plates. The colonies were re-streaked on the same medium and on Difco-Marine Agar to check for purity and bacterial con- tamination. Stock cultures were maintained as slants or plates at 25 °C (12:12) with bimonthly transfers. Morphological and growth studies required transfer of cells from the agar surface to a modified Provasoli's (liquid) medium, followed by gentle glass homogenization. Cell cultures (25 /A) were grown in Terasaki plates containing filter. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
Size: 1889px × 1323px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
