. dan bai zhi zu xue cong xu lie dao gong neng. botany. 214 èç½è´¨ç»å-¦ 22000 22100 22200 22300 22400 22500 22600 22700 22800 å¾ ç³èç½çè´¨è°±å¾ (a) MALDI-TOF (Perseptive Voyager) ï¼ 20pmol ç³èç½ï¼ç¨Poros 50R1 (ç¸å½äº C4åç¸æ§è´¨ï¼æ¸ æ´ï¼Kussmanç-ï¼1997),ç¨2fJ2%TFAæ´è±ï¼80% MeCNå«lOmg/ml è¥å-é ¸ç´æ¥ä¸é¶ï¼ï¼b) ESI-TOF-MS (Micromass). lOOpmol (åæ¹ï¼è±çç¨HPLC C4æ±ï¼Aquapore Bu-300 A , PE Biownlee),梯度为0⢠05%TFAè³80%ä¹è å , 30min,èç½å¤§çº¦å¨22minæ´è±ï¼æ£æµæ³¢é¿214nm (主峰å åTFA)ã æ®æ¯ç±åç²åºåçå¤ç³çå¿«åå-è½°å»ï¼FAB)质谱è·å¾çãMALDIåESIè¿ä¸¤ç§ææ¯ éåäºå¨é«ç
. dan bai zhi zu xue cong xu lie dao gong neng. botany. 214 èç½è´¨ç»å-¦ 22000 22100 22200 22300 22400 22500 22600 22700 22800 å¾ ç³èç½çè´¨è°±å¾ (a) MALDI-TOF (Perseptive Voyager) ï¼ 20pmol ç³èç½ï¼ç¨Poros 50R1 (ç¸å½äº C4åç¸æ§è´¨ï¼æ¸ æ´ï¼Kussmanç-ï¼1997),ç¨2fJ2%TFAæ´è±ï¼80% MeCNå«lOmg/ml è¥å-é ¸ç´æ¥ä¸é¶ï¼ï¼b) ESI-TOF-MS (Micromass). lOOpmol (åæ¹ï¼è±çç¨HPLC C4æ±ï¼Aquapore Bu-300 A , PE Biownlee),梯度为0⢠05%TFAè³80%ä¹è å , 30min,èç½å¤§çº¦å¨22minæ´è±ï¼æ£æµæ³¢é¿214nm (主峰å åTFA)ã æ®æ¯ç±åç²åºåçå¤ç³çå¿«åå-è½°å»ï¼FAB)质谱è·å¾çãMALDIåESIè¿ä¸¤ç§ææ¯ éåäºå¨é«çµæ度å°å¯¹å¤§åå-éèå´å ççç©åå-è¿è¡æµå®ï¼å èå®ä»¬å¯ç¨äºæ¢ç´¢æç¢ ç³å¤åç©ï¼ç®åå·²ç»è·å¾äºå¨å¯¹èç½æå¤è½éæ¾çéè¡çåå¤ç³ç»æåææ¹é¢çè¾å¥½æ° æ®ãMALDI-TOFçæºåè¡°åï¼PSD) (Naven et al., 1997)æCIO-ESI-TOFçæºå 碰 æ-诱导解离ï¼Packer and Harrison, 1998)æESI-MS-MS (Oxley, 1998)ç-ææ¯å¯ä»¥ æç¢ç³æé®ãç±äºå¾®å-åæ¯ç»ç®¡æ¶²ç¸è°±çæ¥å£éåäºè¢«å离çç³åºåå½¢å¼ç碰æè¯±å¯¼ç¢ çåæï¼ç®åçµå·é¾çµç¦»ææ¯å·²æ为èç½ç³åºåç»æåæçæä½³éæ©ï¼Bulingame, 1996),ä¸å离å-åææ¯ä¸æ´å¥½çæ£æµæ¹æ³ä¹é´ç»åæ¯è´¨è°±çåå±æ¹åï¼æ们æå°å¨è (a) 22000 22100 22200 22300 22400 22500 22600 22700 22800 è´¨è·æ¯ I 22494 3000 2000 1000. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original S. R. Pennington M. J. Dunn.
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Keywords: ., bookcollectionbiodiversity, bookcontributorli, booksubjectbotany
