. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 422 ARNOLD LAZAROW ET AL. OXYGEN UPTAKE nryj I 02 PER jg PROTEIN PER HOUR ISOTONIC I I I I .05 TONICITY (EQUIVALENTS .10 .15 OF MEDIUM OF NaOf IN MAO .20 FIGURE 3. Effect of tonicity of the medium on the respiration of islet slices suspended in M Na,HPO4—KH2PO4 buffer, pH Each of the points represents the average of 8 to 10 individual determinations. The measurements at zero tonicity were carried out in distilled water and therefore this point represents the respiration in the absence of phosp
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 422 ARNOLD LAZAROW ET AL. OXYGEN UPTAKE nryj I 02 PER jg PROTEIN PER HOUR ISOTONIC I I I I .05 TONICITY (EQUIVALENTS .10 .15 OF MEDIUM OF NaOf IN MAO .20 FIGURE 3. Effect of tonicity of the medium on the respiration of islet slices suspended in M Na,HPO4—KH2PO4 buffer, pH Each of the points represents the average of 8 to 10 individual determinations. The measurements at zero tonicity were carried out in distilled water and therefore this point represents the respiration in the absence of phosphate. this was 5% greater than the activity in M phosphate buffer. Although this difference is slight, it does agree with the value that would be expected on the basis of data shown in Figures 2 and 3. A decrease in the phosphate concentration from M to M would bring about a 3% decrease in the metabolic activity (Fig. 2), whereas a change in the tonicity of the medium from a sodium chloride equivalent of M to M would bring about a 13% increase in the meta- bolic activity. Therefore in the subsequent studies we used a M Na2HPO4- KH,PO4 buffer, pH , which has a tonicity equivalent to M Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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