. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 264 D R LEVITAN Table II Percent ( transformed) eggs tcruliied in free-drifting eggs sv DF SS MS F Distance 3 ** Current 1 NS Error 11 Total 14 ** = P < .01. NS = not significant. Analysis of covariance with one main effect; distance between released eggs and sperm. The current velocity was the covariate. Table III Effect of population density and body size on the percentage of eggs being fertilized SV DF SS MS F Density 2 *** Size 2 1
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 264 D R LEVITAN Table II Percent ( transformed) eggs tcruliied in free-drifting eggs sv DF SS MS F Distance 3 ** Current 1 NS Error 11 Total 14 ** = P < .01. NS = not significant. Analysis of covariance with one main effect; distance between released eggs and sperm. The current velocity was the covariate. Table III Effect of population density and body size on the percentage of eggs being fertilized SV DF SS MS F Density 2 *** Size 2 NS Interaction 4 NS Error 84 *** = p < .001, NS = not significant. Two-way ANOVA of arcsine transformed data (percent fertilization). Factors were male density and male body size. mesh impedes the flow through the containers, while holding the eggs stationary increases the amount of water and sperm sampled by eggs. With the free-drifting eggs, the release of gametes through syringes might increase estimates of fertilization because the sperm were extruded at a high rate (Denny and Shibata, 1989) and the eggs were released and captured in the center of the passing sperm plume. If, on balance, the Nitex method under- estimates fertilization and the free-drifting method over- estimates fertilization, then Figure 1 represents the upper and lower bounds of actual fertilization success at these current velocities. Most importantly, finding the same pattern of decreasing fertilization success with distance, using different techniques, provides the robust conclusion that relative fertilization rates can be assayed in the field. The Nitex bag method is convenient, and provides a clear relative measure of fertilization success. Population density and body size Over natural ranges of population density and body size, male population density had a highly significant effect on the percent of eggs fertilized, while male body size had no significa
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
