. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CELL DETACHMENT IN SYMBIOTIC CNIDARIANS 327. Figure 3. Scanning electron micrographs of individual host cells released by Aiplusia pulchella (A) and Pocillopora damicornis (B) in response to cold stress, and those obtained from .-1 pulchella (C) and P. damicornis (D) by tissue maceration. Bar = 1 urn. stressed or macerated cells and dehydration completed through 90, 95, and 100% (X3) ethanol. The preparations were embedded via propylene oxide into epoxy resin (Spurr). Thin sections were cut using a Sorvall 6000 ul- tramicrot
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CELL DETACHMENT IN SYMBIOTIC CNIDARIANS 327. Figure 3. Scanning electron micrographs of individual host cells released by Aiplusia pulchella (A) and Pocillopora damicornis (B) in response to cold stress, and those obtained from .-1 pulchella (C) and P. damicornis (D) by tissue maceration. Bar = 1 urn. stressed or macerated cells and dehydration completed through 90, 95, and 100% (X3) ethanol. The preparations were embedded via propylene oxide into epoxy resin (Spurr). Thin sections were cut using a Sorvall 6000 ul- tramicrotome, stained with lead acetate, and viewed on a JEOL transmission electron microscope. Protein determination To investigate the loss of animal protein to the seawater as a result of temperature stress, the seawater was removed from the Petri dishes of cold stressed and control A. pul- chella and homogenized in a teflon-glass tissue grinder. Sodium dodecyl sulphate (SDS, 1% in seawater) was added to each homogenate to a final concentration of (modified from McAuley, 1986). For P. damicornis a 4 ml sub-sample was removed from 25 ml seawater samples, homogenized, and treated with SDS as described above. Each sample was incubated at room temperature for 45 min to solubilize protein in the seawater and host cell membranes associated with released algae. The algae were pelleted by centrifugation (Damon/IEC model HN-S for 4 min at 3000 rpm) and the supernatant put aside for protein analysis as described below. Each algal pellet was resuspended in a known volume of MFSW and the total number of algae assessed using a hemacytometer. Two 1 ml samples were removed from each supernatant and the amount of protein assessed spectrophotometri- cally using the method of Hartree (1972). To ensure that protein in the seawater samples was animal in origin and. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloratio
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
