. The Biochemical journal. Biochemistry. BACTERIAL DECOMPOSITION OF HISTIDINE 455 C ^ Total increase in ammonia nitrogen under the same conditions; D = Total decrease (or increase) in total non-amino nitrogen (as previously- defined) ; E = Total increase in non-amino nitrogen (as previously defined); the ratios which these values would have in the following hypothetical cases may be considered: (i) Simple de-aminisation of the side chain. If the amino acid nitrogen in the side chain were converted into ammonia, then 40 35 â ^ 30 C 1 D 0 E 2 B~V B~V B~\ _o-~-o D _0 O. 5 \0 15 20 25 30 35 40 45
. The Biochemical journal. Biochemistry. BACTERIAL DECOMPOSITION OF HISTIDINE 455 C ^ Total increase in ammonia nitrogen under the same conditions; D = Total decrease (or increase) in total non-amino nitrogen (as previously- defined) ; E = Total increase in non-amino nitrogen (as previously defined); the ratios which these values would have in the following hypothetical cases may be considered: (i) Simple de-aminisation of the side chain. If the amino acid nitrogen in the side chain were converted into ammonia, then 40 35 â ^ 30 C 1 D 0 E 2 B~V B~V B~\ _o-~-o D _0 O. 5 \0 15 20 25 30 35 40 45 50 Time in days Fig. 7. Experiment VH. B. proteus vulgaris (ii) Simple decarboxylation of the side chain. If this reaction alone took place, no ammonia would be formed. The amino acid nitrogen would decrease, since the amine grouping âCH2âNHg does not give any appreciable amount of nitrogen under the conditions employed for estimating amino acid nitrogen. mi CODIES Then ;g = l, ^-y ^-j. (iii) Production of ammonia from both side chain and nucleus. Since there are two nitrogen atoms in the iminazole nucleus, we may assume that eithei (a) both nuclear nitrogen atoms and side chain nitrogen would be attacked at the same rate, then C _ 3 Z) _ 2 E _() B~V B~V B~V. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Biochemical Society (Great Britain); University of Liverpool. Biochemical Dept. London [etc. ] Cambridge University Press
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