. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PLANARIAN REGENERATION WITH COLCHICINE 61 3. The effect of colchicine on regeneration when ivhole aninilas are treated prior to section The preceding work showed that planarian pieces were inhibited in both reconstitution and regulation of form when exposed to certain concentrations of colchicine. Obviously the colchicine was exerting its effects on tissue which had been stimulated to regenerate by cutting. The problem which then presented itself dealt with the effect of colchicine on unactivated tissue. To test this, un- in
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PLANARIAN REGENERATION WITH COLCHICINE 61 3. The effect of colchicine on regeneration when ivhole aninilas are treated prior to section The preceding work showed that planarian pieces were inhibited in both reconstitution and regulation of form when exposed to certain concentrations of colchicine. Obviously the colchicine was exerting its effects on tissue which had been stimulated to regenerate by cutting. The problem which then presented itself dealt with the effect of colchicine on unactivated tissue. To test this, un- injured entire animals were exposed to various concentrations of the agent for 2-6 days after which they were sectioned into l/6th pieces and permitted to reconsti- tute in water. Because these experiments were performed in summer, all intact animals and pieces were kept on the water table at 17-19° C. The data were taken on the tenth day following section. This experiment included 200 animals. In general, pieces from treated animals behave much the same way as if they, themselves, had been exposed to the agent. That is, there was prolonged inhibition of regulation and blastema formation in the higher concentrations. The most M/300O v i n/7,ooo. FIGURE 2. Types of heteromorphic forms obtained from l/6th pieces of 14—16 mm. animals pre-treated with colchicine different lengths of time (M/5,000 for 4 days; M/7,000 for 6 days). Whole animals and pieces maintained at 17-19° C. important difference between the regenerates obtained here and those from treatment following section occurred in the development of a high percentage (36 per cent at D level) of heteromorphic forms when the whole animals were treated for 4 days with M/5,000 and 6 days with M/7,000 colchicine. Regenerates classified as heteromorphic included: (1) simple bipolars, (2) unregulated bipolars (with or without blastemae), (3) poorly regulated forms with two heads at the anterior end, and (4) poorly regulated form
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
