. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. COPPER, ZINC, AND MT 451 LU — L **n — > OJ COPPER MINUTES I— A 0 CN=2) ^ IB »»« ° 15 (N=3) / \ G 45 (N-4) tr LU °- to / ", • 60 / \ + 9° (N = 4) (N-4) Q_ O / \ ^~^ * CD LU _l 5 / 0 1 0 > /rf,/j ° « 0 QL CJ 4 mfl. ^^k K ^ Q *— SA»AA £L — i iBv^AvAvs Md666666Ai. 10 16 20 25 X 36 40 FRRCTIDN NUMBER BO 66 BO Figure -4. Average elution profiles of protein bound copper and zinc in digestive gland cytosols at five specific times after ecdysis (0, 15, 45, 60, and 90 min). Each elution profile is a computer generat
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. COPPER, ZINC, AND MT 451 LU — L **n — > OJ COPPER MINUTES I— A 0 CN=2) ^ IB »»« ° 15 (N=3) / \ G 45 (N-4) tr LU °- to / ", • 60 / \ + 9° (N = 4) (N-4) Q_ O / \ ^~^ * CD LU _l 5 / 0 1 0 > /rf,/j ° « 0 QL CJ 4 mfl. ^^k K ^ Q *— SA»AA £L — i iBv^AvAvs Md666666Ai. 10 16 20 25 X 36 40 FRRCTIDN NUMBER BO 66 BO Figure -4. Average elution profiles of protein bound copper and zinc in digestive gland cytosols at five specific times after ecdysis (0, 15, 45, 60, and 90 min). Each elution profile is a computer generated average of from 2 to 4 individual crabs. The concentrations of copper and zinc are normalized to the wet weight of the tissue used and to the amount of cytosol applied to the column. in lysosomes via the gut, or via the green gland in the urine. Our functional model of copper detoxification agrees with the observations made by Al-Mohanna and Nott (1989) on the shrimp Penaeus semisulcatus. In their elec- tron microscopic examination of the shrimp hepatopan- creas during the molt cycle, they demonstrated the pres- ence of copper and sulfur containing granules in the R cells of the hepatopancreas using EDAX energy dispersive microanalysis. These granules are released to the lumen of the hepatopancreas through cellular degeneration and sloughing. The occurrence of these copper containing granules may be associated with the synthesis and turnover of hemocyanin. The conversion of the MT back to a copper protein occurs later in the postmolt period, B, and Bi, and pre- D COPPER Q ZINC 15 45 60 TIME HFTER ECDYSIS (MINUTES) Figure 5. Histograms showing the average cytosolic concentrations of copper and zinc (; i'. millimoles/kg) in digestive glands at five times after ecdysis (0. 15, 45, 60, and 90 min). These metal concentrations are calculated from the cytosolic samples applied to the Sephadex G-75 col- ce
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
