. Botanical microtechnique. Botany -- Anatomy; Botany -- Morphology; Microscopy -- Technique; Microscopes -- Technique; Botany; Plants -- anatomy & histology; Microscopy. Infiltration and Embedding in Paraffin Wax 35 triangle in a pan, which serves as an air bath, heated by a small Bunsen flame (Fig. ) . Infiltration With Paraffin Wax The following infiltration procedure may be used with any of the common sohents, if the respective specific gravities of the solvent and the wax are taken into account. Paraffin wax, either in solid or melted form, floats on chloroform and also on dioxan.
. Botanical microtechnique. Botany -- Anatomy; Botany -- Morphology; Microscopy -- Technique; Microscopes -- Technique; Botany; Plants -- anatomy & histology; Microscopy. Infiltration and Embedding in Paraffin Wax 35 triangle in a pan, which serves as an air bath, heated by a small Bunsen flame (Fig. ) . Infiltration With Paraffin Wax The following infiltration procedure may be used with any of the common sohents, if the respective specific gravities of the solvent and the wax are taken into account. Paraffin wax, either in solid or melted form, floats on chloroform and also on dioxan. Therefore, either of these solvents, used alone, provides the progressive infiltra- tion that will be emphasized in this chapter. The addition of chloro- form or xylene to dioxan, as suggested previously, merely accelerates the dissolving of the wax. Paraffin sinks in xylene, but if the melted wax is poured into a bottle of cold xylene along the side of the bottle, a solidified layer of wax will remain on top of the solvent during preliminary infiltra- tion at 25 to 35°C. When the solvent is warmed above the melting temperature of the wax, the nearly pure wax will sink to the bottom and envelop the tissues. The abrupt concentration gradient between. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Sass, John E. (John Eugene), 1897-1971. Ames, Iowa State College Press
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Keywords: ., bookcentury1900, booksu, booksubjectbotany, booksubjectmicroscopy
