. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 272 A. C. BROWN AND N. B. TERWILLIGER ibration for measurement of each ion species was done with salt solutions of known concentration spanning the expected range of values. Data analysis Results are expressed as mean ± (n = number of observations). Three-way analysis of variance (ANOVA) was used to test for significance among treatments (de- velopmental stage, salinity, and temperature). Subsequent multiple comparisons of means were performed using the Tukey-Kramer method. Statistical significance was ac- cepted at P &
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 272 A. C. BROWN AND N. B. TERWILLIGER ibration for measurement of each ion species was done with salt solutions of known concentration spanning the expected range of values. Data analysis Results are expressed as mean ± (n = number of observations). Three-way analysis of variance (ANOVA) was used to test for significance among treatments (de- velopmental stage, salinity, and temperature). Subsequent multiple comparisons of means were performed using the Tukey-Kramer method. Statistical significance was ac- cepted at P < Results Estuarine salinity and temperature were measured in areas where the different developmental stages of C. mag- ister were abundant in order to set limits for these param- eters in laboratory studies. The tideflat environment of juveniles ranges from 10°C at high tide to 25 °C when the tide has receded and tideflats are exposed during early- to mid-morning low tides in summer. At the same time, salinity drops from 32 to 16%o as the freshwater lens on the surface passes down the flats. In the channels where adults are found, summer water temperature (10-15°C) and salinity (32-20%o) are much more stable. Winter water temperature is consistently low (10-12°C). Winter range of salinity (32-16%o) at depth in the estuary varies as widely as salinity on the summer tideflats owing to the increased fresh water input from rain. Osmoregulation After 8-h exposure to 100% seawater, the megalopa, 1st instar juvenile, 5th instar juvenile, and adult are isos- motic with the ambient seawater (Fig. 1). In 75% seawater, the hemolymph osmolalities of all four stages are signif- icantly lower than in 100% seawater, yet they are all hy- perosmotic relative to 75% seawater. In 50% seawater, the hemolymph osmolalities of all four stages are significantly lower than in 75% seawater, and all are significantly hy- perosmotic compared with 50% seawater. The 1st juvenile is least able
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