. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. E s 10 - 10 15 20 25 Fraction Number 30 35 Figure 1. Elution profile of the blood cell homogenate of Ascidia gemmata on Sephadex G-15 column chromatography. Pelleted blood cells ( g) were homogenized in about 50 mM HCI buffer solution, which was adjusted at pH The homogenate (14 ml) was loaded onto a column ( cm 0 x 48 cm) and eluted with the same solution in 5-ml fractions. The total bed volume (Vt) and the void volume (Vo) of the column were 85 ml and 39 ml, respectively. The elution volume (Ve) of vanadobin wa
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. E s 10 - 10 15 20 25 Fraction Number 30 35 Figure 1. Elution profile of the blood cell homogenate of Ascidia gemmata on Sephadex G-15 column chromatography. Pelleted blood cells ( g) were homogenized in about 50 mM HCI buffer solution, which was adjusted at pH The homogenate (14 ml) was loaded onto a column ( cm 0 x 48 cm) and eluted with the same solution in 5-ml fractions. The total bed volume (Vt) and the void volume (Vo) of the column were 85 ml and 39 ml, respectively. The elution volume (Ve) of vanadobin was 65 ml. tained in fractions 199 through 216. The amount of va- nadium recovered was ng. The peak fraction (fraction 205) exhibited absorption maxima at 236 nm and 756 nm, as shown in Figure 4. A. gemmata was the only species in which vanadobin exhibited absorption peaks in the visible range. Absorption spectra ofvanadium(III) sulfate and vanadiwn(IV) oxide sulfate Absorption spectra of vanadium(III) sulfate and va- nadium(lV) oxide sulfate are shown in Figures 5a and b, respectively. The former had absorption peaks at 400 nm and 610 nm, and the latter a peak at 760 nm with a shoul- der at 625 nm. When vanadium(III) sulfate (V:(SO4),) 20. 03 0? 300 400 500 600 WavKength(nm) 600 Figure 2. Absorption spectrum of vanadobin eluted from Sephadex G-15 column chromatography. The spectrum was recorded for the peak fraction 12 shown in Figure 1. Absorbance at 245 nm, 410 nm. and 756 nm with a shoulder 620 nm was Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Ma
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