. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Xanthene Dyes 183 q30 FAST ACID BLUE R C. I. NO. 760 Almost the same as: Violamine SB. N_/"~\_0 CH. CH3. CseHaeNaOgSNazCU; MoL Wt. {An acid dye; absorption maximum 53^) The dye indexes (both Schultz and the Colour Index) list vi- olamine 3B as almost synonymous with this. Violamine 3B, how- ever, is a mixture containing a small amount of some red dye. Some samples of fast acid blue R, made for textile application, ar


. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Xanthene Dyes 183 q30 FAST ACID BLUE R C. I. NO. 760 Almost the same as: Violamine SB. N_/"~\_0 CH. CH3. CseHaeNaOgSNazCU; MoL Wt. {An acid dye; absorption maximum 53^) The dye indexes (both Schultz and the Colour Index) list vi- olamine 3B as almost synonymous with this. Violamine 3B, how- ever, is a mixture containing a small amount of some red dye. Some samples of fast acid blue R, made for textile application, are also mixtures, although pure samples have been obtained. It is hoped that pure dye of this type may become regularly available for use as a biological stain. This dye has been recommended by Romell for use in phenolic solution for staining bacteria in soil. In the case of soils (mucks, forest soils, etc.) containing much brownish or reddish matter, this blue dye gives greater visibility of the microorganisms than dyes of the eosin series. In practice the rhodamines are prepared not from xanthene but by the condensation of two molecules of dialkyl meta-amino phenols, R2N / \_0H \_/ with one of phthalic anhydride: CO \ O . / CO This shows their close relation to the next group of dyes, namely the fluoran derivatives, which as will be seen are also prepared from phthalic anhydride. In fact these two groups of dyes, acid and basic respectively, are related in exactly the same way as the rosolic acids and the rosanilins, the one group having hydroxyl radicals where the other has amino Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975. Geneva, N. Y. , Biotech Publications


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