. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CENTRIOLES IN SKATE ERYTHROCYTES 345 TABLE I Distribution ofcentrioles in erythrocyte cytoskeletons prepared from different animals (phase contrast observations, oil immersion) Skate No. cytoskeletons countedb No. with centrioles adjacent to MBC % Adjacent 1 104 70 67 2 107 65 60 3 100 80 80 4 100 52 52 5 103 52 50 6 100 55 55 a Blood samples were taken from skates during summer months at the MBL, except for #5, which was maintained at Hunter College at 10°C without feeding for more than two months before use. b Triton
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CENTRIOLES IN SKATE ERYTHROCYTES 345 TABLE I Distribution ofcentrioles in erythrocyte cytoskeletons prepared from different animals (phase contrast observations, oil immersion) Skate No. cytoskeletons countedb No. with centrioles adjacent to MBC % Adjacent 1 104 70 67 2 107 65 60 3 100 80 80 4 100 52 52 5 103 52 50 6 100 55 55 a Blood samples were taken from skates during summer months at the MBL, except for #5, which was maintained at Hunter College at 10°C without feeding for more than two months before use. b Triton lysis medium was used to prepare cytoskeletons for skates #1-5. For skate #6, Triton lysis medium containing glutaraldehyde was employed to achieve simultaneous lysis and fixation (see Materials and Methods). c Criterion: at least one centriole of the pair within or touching outer zone of cytoskeleton (see Fig. 9). pairs were within or touching an "outer zone" adjacent to the MB, representing less than 25% of the open area (excluding nucleus) of a cytoskeleton. Since the surface of these cells (as viewed on edge) tends to taper as it approaches the narrow perimeter containing the MB, the volume represented by the "outer zone" (Fig. 9) is actually less than 25% of that available. It was notable that in one of the skates, 80% of the centriole pairs were partially or wholly within this area (#3, Table I).. outer zone FIGURE 9. Diagram illustrating the counting procedure used to determine centriole distribution as reported in Table I. In addition to centrioles in contact with the MB, those within or touching the "outer zone" of a cytoskeleton were scored as adjacent to the MB (examples labelled "yes"); those in the interior ("inner zone") were scored as not MB-associated (examples labelled "no"). The "outer zone" was approxi- mately one centriole pair ( nm) in width, representing <25% of open area within
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
