. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 304 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS. a. b. Wound Surface Proximal Epidermis Figure 1. Repeated measurements of ionic current densities following transection. Both traces show repeated measurements made over a 6- min interval at the wound surface and proximal epidermis, a. 15 min post amputation, b. 1 h post amputation. Deflections below the baseline (arrows) indicate an outward current: deflections above the baseline cor- respond to an inward current. Reference bar = 10 a mouth reforms; then the fil
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 304 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS. a. b. Wound Surface Proximal Epidermis Figure 1. Repeated measurements of ionic current densities following transection. Both traces show repeated measurements made over a 6- min interval at the wound surface and proximal epidermis, a. 15 min post amputation, b. 1 h post amputation. Deflections below the baseline (arrows) indicate an outward current: deflections above the baseline cor- respond to an inward current. Reference bar = 10 a mouth reforms; then the filaments elongate, secondary branches appear, and the missing anterior portions of the circulatory and nervous systems differentiate. Extensive cell di- vision is involved in blastema formation and subsequent differ- entiation (8). We have used the voltage-sensitive vibrating probe technique to monitor temporal changes in the steady state ionic currents that are associated with injury, wound healing, and early regen- eration in sabellid fanworms. The probe is a platinum black- tipped insulated wire electrode that responds, relative to a ref- erence electrode, to voltage gradients produced by steady state ion movements. Probes were independently calibrated against a KCl-filled current electrode (6). Amputations and measure- ments were made in filtered seawater at room temperature (21- 22°C). Anesthesia was not used. Immediately following transection, we sometimes detected a strong inward current which declined rapidly and, within 5 min or less, reversed directionality. The maximum inward current that we have measured to date with a one-dimensional vibration system is 40 /lA/cnr. Frequently this inward current was not detected, probably because of its transient nature and the time required to position the amputation surface appropriately to allow data collection. The inward current was replaced by a persistent outward current leaving the wound site. Sabellids are segmented worms.
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
