. Biological structure and function; proceedings. Biochemistry; Cytology. 124 BRIXTON CHANCE suggesting that only ATP is required for pyridine-nucleotide reduction. These considerations eliminate hypotheses which postulate that DPN- linked substrates are acting directly or through an ATP-activated step, since succinate or ATP alone should cause the observed effects. A remarkable feature of the reaction and one which seems to differ considerably from the results so far obtained by Klingenberg and Azzone (this meeting) is the low concentration of ATP required for pyridine- nucleotide reduction.
. Biological structure and function; proceedings. Biochemistry; Cytology. 124 BRIXTON CHANCE suggesting that only ATP is required for pyridine-nucleotide reduction. These considerations eliminate hypotheses which postulate that DPN- linked substrates are acting directly or through an ATP-activated step, since succinate or ATP alone should cause the observed effects. A remarkable feature of the reaction and one which seems to differ considerably from the results so far obtained by Klingenberg and Azzone (this meeting) is the low concentration of ATP required for pyridine- nucleotide reduction. Under favourable conditions it has been observed Fluorescence excitation 365nn/i Fluorescence measurement 450m/i lOmM succinate PH. M + ==jf_' State 1. Fig. 6. Fig. 6. Illustrating separation of electron and energy-transfer require- ments for succinate-linked pyridine- nucleotide reduction. Addition of succinate alone causes no fluores- cence change, while loo jum ATP causes a large fluorescence increase corresponding to pyridine-nucleo- tide reduction. Pigeon-heart mito- chondria suspended in sucrose- " tris "-mannitol medium, pH 7-4, temperature 26° (Expt. 133d). (Reproduced with permission of The Journal of Biological Chemistry.) Fluorescence excitation 365m/i Fluorescence measurement 450myu TS/jM ATP 4mM succinate Mv»_>: (state 1). Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
Size: 1731px × 1444px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookcollectionbiodiver, booksubjectbiochemistry
