. The Biochemical journal, 1918. Biochemistry. 410 A. HARDEN AND S. S. ZILVA A, B, and C for the fat-soluble A, antineuritic, and antiscorbutic factors respectively. The rats were kept separately in cages; couples belonging to the same group were brought together only occasionally for mating purposes. The food was supplied to them fresh in balls every day and the remains of the previous day were weighed daily. The drinking water was changed twice daily. 160 105. 1 2 3 6 Fig. 1. Weight curves of 3 rats on an AC diet. Fig. 1 represents the weight curve of 3 rats kept on an AC diet. The daily
. The Biochemical journal, 1918. Biochemistry. 410 A. HARDEN AND S. S. ZILVA A, B, and C for the fat-soluble A, antineuritic, and antiscorbutic factors respectively. The rats were kept separately in cages; couples belonging to the same group were brought together only occasionally for mating purposes. The food was supplied to them fresh in balls every day and the remains of the previous day were weighed daily. The drinking water was changed twice daily. 160 105. 1 2 3 6 Fig. 1. Weight curves of 3 rats on an AC diet. Fig. 1 represents the weight curve of 3 rats kept on an AC diet. The daily ration of each of these rats was made up as follows: Basal mixture ... ... ... ... 10 g. Antiscorbutic equiv. to lemon juice ... 5 cc. Centrifuged butter fat ... ... ... 1*8 g. Olive oil 1*6 g. The above ingredients were incorporated in a single ball, the olive oil being added in order to obtain a convenient consistency in the paste. From |-| of the daily ration was consumed by the animals. It is seen from the weight curves that none of the animals has gained appreciably in weight during any period of the experiment. This becomes more marked as the rats were at an age when development under normal conditions progresses rapidly. After about three weeks the animals commenced losing in weight and died five weeks after the commencement of the experiment. Rat No. 8 was killed by the buck after 4| weeks when left with it in a cage for mating purposes. Rats Nos. 7 and 9 showed decided neuritic lesions before death. Rat No. 7 first showed marked paralysis in the hind limbs four days before death. This condition became gradually worse. Rat No. 9 showed even more striking symptoms. The following was its condition three. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Biochemical Society (Great Britain); University of
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