. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 30 -r 0 60 0 30 60 H H. .o- .-o- 30 60 30 60 FIGURE 2. Time course of lactic acid production by anaerobic homogenates. Clutch Solid lines, R. pipicns. Broken lines, hybrids. Substrate, glycogen. Upper left, standard age 4 hours. Upper right, standard age 56 hours. Lower left, standard age 84 hours. Lower right, standard age 140 hours. Abscissae, minutes. Ordinates, //g. lactic acid per embryo, 24° C. Homogenized embryos: 10 per nil. Yeast hexokinase was added in some experiments (see RESULTS, Gl
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 30 -r 0 60 0 30 60 H H. .o- .-o- 30 60 30 60 FIGURE 2. Time course of lactic acid production by anaerobic homogenates. Clutch Solid lines, R. pipicns. Broken lines, hybrids. Substrate, glycogen. Upper left, standard age 4 hours. Upper right, standard age 56 hours. Lower left, standard age 84 hours. Lower right, standard age 140 hours. Abscissae, minutes. Ordinates, //g. lactic acid per embryo, 24° C. Homogenized embryos: 10 per nil. Yeast hexokinase was added in some experiments (see RESULTS, Glucose) to a final concentration of mg. (27 KM units) per ml. The enzyme and all co- factors and substrates, excepting glucose, were obtained from Mann Research Laboratories, Inc., New York City, and were made up in fresh solutions for each experiment. Anaerobiosis was obtained with the help of the apparatus previously described (Greeg, 1962), consisting of a train of flasks, each containing 2 ml. of the homoge- nate system, continuously flushed with 95% N.,:5% CO2 and maintained in a shaking bath at 24° C. At the ends of suitably chosen intervals, flasks were re- moved from the distal end of the train, ml. of 30% trichloroacetic acid added to. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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