AMAarchives of neurology & psychiatry . rom the brain filtrate of the animals that died. Some of the cultures were carried through twenty-two were repeatedly filtered and the minute organism recoveredfrom the filtrate. Spinal fluid from the following control cases and brain filtrates fromall but the last two were inoculated intracranially into thirty rabbitswith negative results: three cases of glioma of brain, one case each of 296 /IRC IIIrUS ()l= AM) ISYCIIIAiKV tiil)ercul()us nienin,t,ntis (verified at necr()]).sy), J [fxlj^kins disease,poliomyelitis, stapli
AMAarchives of neurology & psychiatry . rom the brain filtrate of the animals that died. Some of the cultures were carried through twenty-two were repeatedly filtered and the minute organism recoveredfrom the filtrate. Spinal fluid from the following control cases and brain filtrates fromall but the last two were inoculated intracranially into thirty rabbitswith negative results: three cases of glioma of brain, one case each of 296 /IRC IIIrUS ()l= AM) ISYCIIIAiKV tiil)ercul()us nienin,t,ntis (verified at necr()]).sy), J [fxlj^kins disease,poliomyelitis, stapliylococcus bacteremia, tubercle of cord (verifiedmicroscoi)ically) and diabetes. Cultures on ordinary mediums of tbese s])inal fluids and brainfiltrates yielded no t^rowtb. Most of tbe sj)inal fluids, brain filtratesand filtered nasojiharyng^eal wasbin,t(s were similarly cultivated andsbowed no growtb, excej)t an occasional spinrd fluid from wliicb anobvious contamination was recovered. Filtrates from tbe rabbit brainsyielded no Fig. 9.—This illustration shows the comparative size of Staphylococcusaureus and the minute filtrable organism cultivated in Noguchi medium fromthe brain filtrate of a case of epidemic encephalitis; X 1200. Altbougb tbe buman brains were not removed aseptically atnecropsy, nevertheless two brains were cultivated on ordinary mediums(including blood agar) and in deep tubes (wbich had been autoclaved)of glucose bouillon containing a piece of sheep brain. The surface ofthe brain was seared and pieces were removed aseptically from the cere- THALHIMER—EPIDEMIC EXCEPHALITIS 297 brum and midbrain. Seven rabbit brains, removed aseptically, weresimilarly cultivated. Most of the tubes inoculated with the humanbrains showed growths of saprophytic bacteria. Only an occasionaltube inoculated with rabbit brain yielded growth, usually Staphylococcusaureus. Streptococci (hemolytic or anhemolytic) were never recoveredfrom these blocks of brain, brain filtrate
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