. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 92 B. VERACHTERT AND A. DE LOOF Conway, 1941). NaCl mM, NaH:PO4 1 mM NaHCO3 10 mM. MgCl2 mM. CaCl2 mM. CaSO4 mM. MgSO4 mM, Na:SO4 mM K2SO4 30 mM, sucrose 127 mM; 95% O2/5% CO2; Manduca sexta KC1 40 mM, MgCl2 15 mM. CaCl: 5 mJl/and Tris- succinate 110 mM, pH Pressure injection We used an Eppendorf SL42 Microinjector System to inject lucifer yellow (3% inaquadestillata). The injection pipette was mounted on a hydraulic micromanipulator (Narishige MO-108). Volumes of 10-30 pi were injecte
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 92 B. VERACHTERT AND A. DE LOOF Conway, 1941). NaCl mM, NaH:PO4 1 mM NaHCO3 10 mM. MgCl2 mM. CaCl2 mM. CaSO4 mM. MgSO4 mM, Na:SO4 mM K2SO4 30 mM, sucrose 127 mM; 95% O2/5% CO2; Manduca sexta KC1 40 mM, MgCl2 15 mM. CaCl: 5 mJl/and Tris- succinate 110 mM, pH Pressure injection We used an Eppendorf SL42 Microinjector System to inject lucifer yellow (3% inaquadestillata). The injection pipette was mounted on a hydraulic micromanipulator (Narishige MO-108). Volumes of 10-30 pi were injected into either the oocyte or the nurse cells of follicles ofSar- cophaga bullata. The injected follicles were examined by epiillumina- tion fluorescence on a Nikon diaphot-TMD inverted mi- croscope with the appropriate filter cassette. Vibrating probe The extracellular electrical field was measured using a one dimensional vibrating probe (Jaffe and Nuccitelli, 1974) with parylene coated metal electrodes. Some ex- periments were performed with a two-dimensional vi- brating probe set up at Woods Hole. The measurements were performed in a small dish filled with Ringer solution (1 ml). The Ringer solutions were exchanged with syringes while the follicles re- mained at the same place. After exchange, the probe was allowed to equilibrate for at least 20 min. Results and Discussion Dye injection When lucifer yellow was pressure injected in either the oocyte or the nurse cell compartment of a follicle ofSar- cophaga bullata, the dye quickly spread throughout the whole follicle in less than 15 min. Sometimes, a few nurse cells were excluded from being stained. Similar ob- servations have been made on Drosophila melanogaster and Manduca sexta vitellogenic follicles (Fig. 1.). The spread of lucifer yellow from one germ cell (oo- cyte or nurse cells) to another occurred via the intercellu- lar bridges or ring channels between the cells. These ring channels form large cytoplasmic connections
Size: 1039px × 2404px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
