. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FERTILIZATION AND DEVELOPMENTAL BIOLOGY 295 1A. Survival of Medaka Embryos Injected With TCDD Time After Iniecnon •« 24 Hrs 48 Hi-. Hcjrl 1V|££1 72 Mrs 96 Mrs Conccn( N 1(1 ppm 4 r i Hi",) 0 0 0 ) 1 pprn 32 18 i 561 I 13 (411) 2 (61' I 13 I4l'il 1 i II' 001 ppm : i |96", > 23 (96't) 1 (3"_'•, 11 (9211 0 11 (9211 II 92'.",, 1 1B. Figure 1. (A) I 'iahilily of medaka embryos injected with TCDD at the guerilla stage (24 h poxt fertilisation). Medaka hatch at approximately 14 davs (lil I'liniiiin
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FERTILIZATION AND DEVELOPMENTAL BIOLOGY 295 1A. Survival of Medaka Embryos Injected With TCDD Time After Iniecnon •« 24 Hrs 48 Hi-. Hcjrl 1V|££1 72 Mrs 96 Mrs Conccn( N 1(1 ppm 4 r i Hi",) 0 0 0 ) 1 pprn 32 18 i 561 I 13 (411) 2 (61' I 13 I4l'il 1 i II' 001 ppm : i |96", > 23 (96't) 1 (3"_'•, 11 (9211 0 11 (9211 II 92'.",, 1 1B. Figure 1. (A) I 'iahilily of medaka embryos injected with TCDD at the guerilla stage (24 h poxt fertilisation). Medaka hatch at approximately 14 davs (lil I'liniiiinicrograph of 96 h post fertilisation (lipfl sehra/ixli that was dechorionated at the gastnda stage ( hp/) and exposed to 1011 tig/ml TCDD for 30 min. Antibody staining demonstrates CYP1A activation in the epithelial cells of the liver (large arrow) and stomach I small arrowxl. t V//.v lining the gills. pharyn.\. ami esophagus leading into the stomach are devoid of stain. Y = yolk; G = gi/lx; E = eye. Mag- nification htir = mm. CYP1A monooxygenases through binding of TCDD to the aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor (11). Staining of these TCDD-treated embryos with a monoclonal antibody prepared against fish CYP1A (MAb 1- 12-3) showed that CYP1A was already present and activated in these 96-h-old zebrafish and newly hatched medaka; expression was seen in multiple organs of both species. At the time of sam- pling for analysis, this staining was seen prominently in endo- thelial cells of multiple vascular structures, and in the epithe- lium of various organs including liver, gut, and kidney, (Fig. IB). CYP1A activates or inactivates many foreign compounds; indeed CYP1A and the AhR both might participate in the tox- icity of TCDD and related compounds (12). Recent studies! 13) have suggested that CYP1A may be causally linked to the effects of TCDD in medaka embryos, possibly through stimu- lating the release of reactive oxygen speci
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
