. Ciba Foundation colloquia on ageing. Old age; Aging; Animals -- growth & development. 220 Discussion DISCUSSION Zweymiiller: The identification of organic acids in urine by paper chromatography is elegant and of general application. The Rp values of the different organic acids are distinctly different and therefore a clear separation on the paper is possible. We used the technique developed by Nordmann and co-workers (1954. Acad. Sci., Paris, 238, 2459), and Fig. 1 demonstrates the position on a two-dimensional descending chromatogram of some non-volatile, water-soluble organic acid
. Ciba Foundation colloquia on ageing. Old age; Aging; Animals -- growth & development. 220 Discussion DISCUSSION Zweymiiller: The identification of organic acids in urine by paper chromatography is elegant and of general application. The Rp values of the different organic acids are distinctly different and therefore a clear separation on the paper is possible. We used the technique developed by Nordmann and co-workers (1954. Acad. Sci., Paris, 238, 2459), and Fig. 1 demonstrates the position on a two-dimensional descending chromatogram of some non-volatile, water-soluble organic acids which. 05 Et OH-NH3-H2O Fig. 1 (Zweymiiller). The position of some organic acids in the urine of a normal adult on a two-dimen- sional descending chromatogram. Ci = Citric acid, Ta = Tartaric, Ma = Malic, Gly = Glycolic, a-ce = a-ketoglutaric, Su = Succinic, Ac ^Aconitic, Glu = Glutaric, p-hy = [B-hydroxy- butyric, La = Lactic, Hi = Hippuric. Nordmann has found in the urine of normal adults. There is clear separa- tion of citric acid, tartaric, malic, a-ketoglutaric, succinic, aconitic, lactic, glycolic, hippuric, glutaric and p-hydroxybutyric acids. One spot applies to both sulphate and phosphate, if there is any phosphate in the urine. Using this method the organic acids give yellow spots on a blue-greenish background. These spots have the advantage that they do not fade but get more intense with time. We have so far examined urines passed by newborn babies on the first, second and seventh days of life, but we have not done enough to give a complete answer yet. Citric acid appears to be. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation; Wolstenholme, G. E. W. (Gordon Ethelbert Ward). Boston, Little, Brown
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