. The principles of bacteriology: a practical manual for students and physicians. ound that by tlie use of a particular stainingmethod the appearance of bacillus diphtherias is strik-ingly unlike that of the confusing forms. His differ-ential method comprehends the following manipulations:the culture to be tested should be grown upon Lofflersblood-serum mixture solidified at 100° C.; it should Neisser : Zeitschrift fiir Hygiene und Infektionskraukheiten, 1897,Bd. xxiv. PSE UD 0-DIPHTHERITIG BA GILL US. 403 develop at a temperature not lower than 34° C. and nothigher than 36° C.; and it should
. The principles of bacteriology: a practical manual for students and physicians. ound that by tlie use of a particular stainingmethod the appearance of bacillus diphtherias is strik-ingly unlike that of the confusing forms. His differ-ential method comprehends the following manipulations:the culture to be tested should be grown upon Lofflersblood-serum mixture solidified at 100° C.; it should Neisser : Zeitschrift fiir Hygiene und Infektionskraukheiten, 1897,Bd. xxiv. PSE UD 0-DIPHTHERITIG BA GILL US. 403 develop at a temperature not lower than 34° C. and nothigher than 36° C.; and it should be not younger than nineand not older than twenty-four hours. A cover-glass prep-aration made from such a culture is stained as follows: a. It is subjected to the following mixture for fromone to three seconds: Methylene-blue (Griiblers) 1 gramme. Alcohol (96 per cent.) 20 When dissolved, mix with Acetic acid . 50 Distilled water . . 950 b. After thoroughly rinsing in water, it is stained forfrom three to five seconds in vesuvin (Bismarck-brown), Fig. BacUlue diphtkerise, stained by Neissers method. 2 grammes, dissolved in 1 litre of boiling distilled water,filtered, and allowed to cool. It is again rinsed in water 404 BACTERIOLOGY. and examined as a water-mount, or it may be dried andmomited in balsam. When so treated the diphtheria bacillus appears asfaintly stained brown rods, in which from one to threedark-blue granules are always to be observed. Thedark granules are at one or both poles of the cell, aremore or less oval, and usually seem to bulge a littlebeyond the contour of the bacillus in which they arelocated. (See Fig. 70.) From Neissers observationsand those of others/ as well as from personal experience,it seems safe in the vast majority of cases to regard allbacilli that do not stain in the way described as distinctfrom bacillus diphtherice.^ Note.—Prepare cover-slip preparations from themouth-cavities of healthy individuals and from thoseh
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