. The Biochemical journal, 1918. Biochemistry. ANTISCOIilUITU; FACTOU IN IJvMoN .UK K â¢-'().â delayed .slightly l)ut not prevented the onset of scurvy. \h tlie doses given were comparatively high it is plain that by drying the treated lemon juice in the way we have just described the best part of its antiscorbutic activity is destroyed. Expl. 5. From experimental evidence at our disposal obtained in the course of another investigation we decided to modify the process of desiccation by previously acidifying the treated lemon juice. After the precipitation of the free acids of the lemon juice w
. The Biochemical journal, 1918. Biochemistry. ANTISCOIilUITU; FACTOU IN IJvMoN .UK K â¢-'().â delayed .slightly l)ut not prevented the onset of scurvy. \h tlie doses given were comparatively high it is plain that by drying the treated lemon juice in the way we have just described the best part of its antiscorbutic activity is destroyed. Expl. 5. From experimental evidence at our disposal obtained in the course of another investigation we decided to modify the process of desiccation by previously acidifying the treated lemon juice. After the precipitation of the free acids of the lemon juice with CaCOg and alcohol 1 gm. of citric acid was dissolved in the alcoholic filtrate of I litre of lemon juice. It was then evaporated in vacuo at 30°-40° as before. A residue of 11 gnis. was obtained. 500. Guinea pigs Nos. 243,244 and 245 received 45mgnis., loOmgnis. respectively besides the usual scorbutic diet. As before the dry residue was dissolved before administration. As will be seen from fig. 5 which represents the weight curves of these animals they were all increasing in weight. After eighty-three days the animals were chloroformed and at the post mortem examination were found to be in excellent condition. Not the slightest signs of scurvy were to be observed. It is thus seen that by acidifying shghtly the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Biochemical Society (Great Britain); University of Liverpool. Biochemical Dept. London [etc. ] Cambridge University Press
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