. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Controls 10'8 M 10'7M 10'6M 10'5M 10JM 10'3M 10'2M Concentration do not mediate responses of larvae of the serpulid poly- chaete Hydroides elegans to metamorphic cues. If cues for settlement and metamorphosis of larvae of H. elegans were bound by a G protein-coupled receptor, we would have expected competent larvae to (1) meta- morphose when exposed to the GTP analog Gpp[NH]p. and (2) fail to respond to inductive bacterial biofilms when those biofilms were presented in combination with GDP-/J-S, a competitive inhibitor of G
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Controls 10'8 M 10'7M 10'6M 10'5M 10JM 10'3M 10'2M Concentration do not mediate responses of larvae of the serpulid poly- chaete Hydroides elegans to metamorphic cues. If cues for settlement and metamorphosis of larvae of H. elegans were bound by a G protein-coupled receptor, we would have expected competent larvae to (1) meta- morphose when exposed to the GTP analog Gpp[NH]p. and (2) fail to respond to inductive bacterial biofilms when those biofilms were presented in combination with GDP-/J-S, a competitive inhibitor of G protein activation by GTP. We observed no increase in metamorphosis over FSW control treatments in the presence of Gpp|NH]p (Fig. 1 A), and no inhibition of response of larvae to bio- filmed surfaces when exposed to GDP-/3-S (Fig. IB). These results imply that the receptors responsible for de- tecting the metamorphic cue in H. elegans are not associ- ated with G proteins. The absence of evidence for the involvement of G pro- tein-coupled receptors does not exclude the possibility that the metamorphic signal is transduced by either the B. en 'en O "§- O CD CD CL 100 - 90 - 80 - 70 - 60 50 40 30 20 10 0. Controls 10'6M 10'5M 10'" M Concentration 10"JM Figure 2. Metamorphosis of larvae of Hydroides elegans exposed to the phosphodiesterase inhibitors IBMX, theophylline, and papaverine. (A) and (B) present results from two separate trials. Note that the con- centration ranges (x axes) differ between trials. Points represent means of 5 replicates in (A), 4 replicates in (B); error bars are standard devia- tions. * - significantly different from the FSW control treatment at P == Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biologica
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
