. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FAT MKTAMOUSM OK CHICK KMBRYO 57 In these experiments two eggs were boiled for 20 minutes, the coagulated yolk was weighed, then dried to constant weight in a Freas electric vacuum oven at 55° C. and vacuum at about cm., ground and subjected for 48 hours to extraction of fat with anhydrous ether by a Soxhlet extraction apparatus. From two or more unboiled eggs the embryos were separated for determination of dry matter and fat content by methods similar to those described above. The extracted fat from both the embryo and
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FAT MKTAMOUSM OK CHICK KMBRYO 57 In these experiments two eggs were boiled for 20 minutes, the coagulated yolk was weighed, then dried to constant weight in a Freas electric vacuum oven at 55° C. and vacuum at about cm., ground and subjected for 48 hours to extraction of fat with anhydrous ether by a Soxhlet extraction apparatus. From two or more unboiled eggs the embryos were separated for determination of dry matter and fat content by methods similar to those described above. The extracted fat from both the embryo and the yolk sac was tested for saponification number (Koettstorfer, 1879), iodine value (\Vijs, 1898), L and refractive index, by a Zeiss butter refractometer. h X cu o o Lmbiyo Egg YoU. J X r+- a -I Days 0 2 H 6 8 10 12 \H 16 18 20 Incubcition Age FIG. 1. Changes in the fat (ether extract) content of the embryo and egg yolk during incubation. RESULTS AND DISCUSSIONS It has been found that the fat content of a fresh fowl's egg is directly proportional to its size. For that reason the amounts of fat obtained from an egg by previous workers can hardly be compared, 1 Both saponification number and iodine value were determined after the methods given in Official and Tentative Methods of Analysis of the Association of Official Agri- cultural Chemists, third edition, 1930, on pp. 321-322. 6. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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