. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . pplication of PlatingMethods.) Growth on Agar and Other Solid Media {Surface Growth).—Moist or dry,flat or raised, flaky or easily emulsified, adherent to medium or riot, mucoidor slimy, smooth, irregular, or threaded margin, color or pigment production(see below), extension into medium, etc. Growth in Solid Media.—In stab culture, depth to which growth extends,character of growth around the surface puncture, the spreading of the growthinto the medium (an index of motility best shown in semisolid m
. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . pplication of PlatingMethods.) Growth on Agar and Other Solid Media {Surface Growth).—Moist or dry,flat or raised, flaky or easily emulsified, adherent to medium or riot, mucoidor slimy, smooth, irregular, or threaded margin, color or pigment production(see below), extension into medium, etc. Growth in Solid Media.—In stab culture, depth to which growth extends,character of growth around the surface puncture, the spreading of the growthinto the medium (an index of motility best shown in semisolid media), thread-like extensions from the hne of punctme, in gelatin, liquefaction, non-lique-faction, rate, area and character if present. In shake cultures, the characterof the colonies. Fluid Media.—Pellicle, tenacious or easily broken, spreading up the side ofthe tube or not, thick or thin, clear or cloudy, and degree of clouding, sedi-ment or deposit on sides of tube, granular, flaky, gelatinous, mucoid, orstringy, stalactite formation, more densely clouded at one level than Fig. 64.—Stab cultures of three cholera spirilla in gelatin, showing in upper portion ofgrowth considerable liquefaction of nutrient gelatin. Food Requirements.—Growth on simple or complex media, albumins such asserum required, blood or hemoglobin necessary, carbohydrate needed f^r fulldevelopment (example glycerin for tubercle bacillus). Oxygen Requirements.—Growth only in free oxygen, obligate aerobe; growthonly where oxygen is absent (depths of media, etc.), obligate anaerobe; growthunder both conditions, facultative anaerobe (an available carbohydrate isnecessary to elicit the facultative character); growth with a definite but lessenedamount of oxygen (development only in one level of solid media), micro-aero-phihc. This last characteristic may be lost after cultivation for some time onartificial culture media. Optimum Temperature.—Not often used, but may furnish absolute evid
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