. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HSR-OMEGA, A NOVEL HEAT SHOCK GENE 83 67 base 93D leader. hydei leader Figure 5. Recombinant DNA constructs used to analyze the trans- lation of the most conserved small open reading frame (ORF) in hxr- onu'Kti- (A) The 93Xho construct was made by joining a bacterial chloramphenicol acetyltransferase (CAT) gene to DNA from the D hsr-nmega gene. The junction was 30 nucleotides past the termination of the small ORF. The 93XR construct was made by deleting the small ORF from 93Xho. If the small ORF is translated i
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HSR-OMEGA, A NOVEL HEAT SHOCK GENE 83 67 base 93D leader. hydei leader Figure 5. Recombinant DNA constructs used to analyze the trans- lation of the most conserved small open reading frame (ORF) in hxr- onu'Kti- (A) The 93Xho construct was made by joining a bacterial chloramphenicol acetyltransferase (CAT) gene to DNA from the D hsr-nmega gene. The junction was 30 nucleotides past the termination of the small ORF. The 93XR construct was made by deleting the small ORF from 93Xho. If the small ORF is translated in vm>. the ribosomes would not be expected to reinitiate on the CAT gene. Therefore the 93Xho construct would not be expected to direct the synthesis of CAT while the 93XR construct, where the CAT transla- tion start is not blocked, should. When the constructs were stably trans- formed into Dmsophila cultured cells, both constructs yielded abun- dant RNA but only the 93XR CAT gene was translated. (B) The S3- 128 construct was made using the D. hydei hsr-omega gene. In this case the bacterial CAT gene was joined in frame to the conserved ORF so that ribosomes translating the ORF should continue translating into the CAT gene and yield CAT protein that is larger by the size of the ORF peptide. Cells carrying this construct do produce the predicted larger CAT. All of the experiments give strong evidence that the hsr- oiiH'xii ORF is translated in wvo(Fini el at-, 1989). synthesis at any particular time and could link some other cellular process to this level. The Nuclear Transcript, Omega 1 One of the questions that we had about hsr-omega was whether all of the agents that induced the puff were act- ing on the hsr-omega sequences. Puffs usually involve more DNA than is actually transcribed, so a puffin the 93D region might indicate activation of a transcription unit that is not hsr-omega. The question had interesting implications. If the agents that induce the hsr-omega puff, bu
Size: 1770px × 1411px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
