The Journal of biological chemistry . ing the administration ofinsulin. The writer has obtained^ however, delayed actionwith certain pancreatic extracts. These results have been re-ported elsewhere (4). Rabbit 1, Chart I, was killed 23| hoursafter the injection had been made in order to estimate the glyco-gen content of the liver. This substance was completely lackingin that organ. Here again, however, a rabbit which has received 516 Glucokinin insulin may on occasion be found to have little or no glycogen inthe liver. This experiment would have been much more con-vincing if the liver had cont
The Journal of biological chemistry . ing the administration ofinsulin. The writer has obtained^ however, delayed actionwith certain pancreatic extracts. These results have been re-ported elsewhere (4). Rabbit 1, Chart I, was killed 23| hoursafter the injection had been made in order to estimate the glyco-gen content of the liver. This substance was completely lackingin that organ. Here again, however, a rabbit which has received 516 Glucokinin insulin may on occasion be found to have little or no glycogen inthe liver. This experiment would have been much more con-vincing if the liver had contained glj^cogen. The blood sugarcurve, Curve 2, Chart I, taken alone is not at all conclusive. Theresult in the case of Rabbit 3, Chart I, furnishes the most convinc-ing evidence that the extract administered contained a potentprinciple. It will be noted that here there w^as first a markedhyperglycemia followed some hours later bj a decided hypogly-cemia with a subsequent return to the normal blood sugar level. Chart nYeast extract n. Hps. 6 !6 24 32 40 Time Chart II. The result obtained in the case of Rabbit 4, Chart I, like that ofRabbit 2, Chart I, if taken alone, is not conclusive. There w^asno marked hyperglycemia in this instance, while at the end of 17hours there was a slight but definite fall in the level of blood sugar. Results of Experiments with Yeast Extract II. Chart II illustrates some results obtained with yeast extractII. Little or no hj^perglycemia w^as noted in this series of experi-ments. The experiment represented by Curve 2, Chart II, iscomplicated by the fact that a watery extract of fresh liver wasalso administered. The animal died in 47 hours. Rabbit 4 of J. B. Collip 517 this series died during the night before further blood sugar de-terminations were made. It will be noted that there is a con-siderable degree of variation in these experiments between thetime of injection and the time of most marked hypoglycemia. Results of Experiments ivith Yeast
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