. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. The Washed Leukocytes 281 The hands of the patient are washed, and a piece of elastic rubber tubing or some other convenient fillet wound about the thumb or a finger to produce venous congestion. With a convenient lancet (Wright uses a pricker made by drawing a bit of glass tubing or a glass rod to a fine point in the flame) a prick is made about a quarter inch from the root of the nail. From this the blood is permitted to flow into small test- tubes pre
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. The Washed Leukocytes 281 The hands of the patient are washed, and a piece of elastic rubber tubing or some other convenient fillet wound about the thumb or a finger to produce venous congestion. With a convenient lancet (Wright uses a pricker made by drawing a bit of glass tubing or a glass rod to a fine point in the flame) a prick is made about a quarter inch from the root of the nail. From this the blood is permitted to flow into small test- tubes previously filled about three-fourths with per cent, sodium citrate solution. The blood and citrate solution are mixed, and the tubes placed in a centrifuge, balaiiced, and centrifugaUzed until the corpuscles are collected at the bottom of the tube. The citrated plasma is now withdrawn and re- placed with per cent, sodium chloride solution, through which the corpuscles are distributed by shaking. The tubes are now again centrifugalized until the corpuscles are collected, when the saUne is removed care- fully, the last drop from the back of the meniscus. In the cor- puscular mass that remains the leukocytes form a thin creamy layer on the P'ig. 92.—Tube of blood and citrate solu- tion before and after centrifugalizing (Miller).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original McFarland, Joseph, 1868-. Philadelphia and London, W. B. Saunders Company
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1919
