. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 494 JOHN W. MEHL The effect of alkali on these myosins is similar to the effects already reported for cow and rabbit inyosin (11). Measurements of the amount of double refraction were made at constant velocity gradient, as a func- tion of time. The double refraction of flow decreases rapidly at pH 10. A decrease is obtained with octopus myosin at pH 8, the loss being about 50 per cent in 45 minutes. At pH 9 a decrease to 50 per cent of the initial value takes place in about 25 minutes. Essentially the same re- sult was obtai
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 494 JOHN W. MEHL The effect of alkali on these myosins is similar to the effects already reported for cow and rabbit inyosin (11). Measurements of the amount of double refraction were made at constant velocity gradient, as a func- tion of time. The double refraction of flow decreases rapidly at pH 10. A decrease is obtained with octopus myosin at pH 8, the loss being about 50 per cent in 45 minutes. At pH 9 a decrease to 50 per cent of the initial value takes place in about 25 minutes. Essentially the same re- sult was obtained with snail FIG. 2. The amount of double refraction (ne — no) -4- C, as a function of the velocity gradient, ft. •—snail myosin at 25°, nitrogen = X 10"* gram/cc.; O—octopus myosin at 25°, nitrogen = X 10~4 gram/cc.; Q—rabbit myosin at 3°, nitrogen = X 1CT4 gram/cc., from the measurements of von Muralt and Edsall (11). The effect of urea upon the double refraction of flow was also studied. The results obtained are shown in Fig. 3, where the percentage of the original amount of double refraction is given as a function of the time. There is a general similarity between the different myosins, with secondary differences. It is perhaps of some interest that the ray myosin is no less sensitive to the effects of urea than are the other myosins, although the uremia of the elasmobranchs (about 2 per cent urea in marine elasmobranchs) is of the same order of magnitude as the concentrations of urea needed to produce denaturation (about 3—4 per cent).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Rich
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