. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 10. \ 70 72 76 PH • o • o s 70 PH 78 80 82 1 1 1 1 10 po2 FIGURE 2. A. PH dependence of O2 binding by Acila castrensis (•), Nucula proximo. (D), Solemya velum (), and Yoldia limatula (O) Hcs. 20°C, M Tris maleate buffered blood. B. Hill plot of 62 equilibrium of Yoldia limulata (•, pH ) and Acila castrensis (O, pH ) Hcs. gen, although the visual observation could not be confirmed even by microspectro- scopic observation due to the very small volume (ca., 10 n\) obtained. O2


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 10. \ 70 72 76 PH • o • o s 70 PH 78 80 82 1 1 1 1 10 po2 FIGURE 2. A. PH dependence of O2 binding by Acila castrensis (•), Nucula proximo. (D), Solemya velum (), and Yoldia limatula (O) Hcs. 20°C, M Tris maleate buffered blood. B. Hill plot of 62 equilibrium of Yoldia limulata (•, pH ) and Acila castrensis (O, pH ) Hcs. gen, although the visual observation could not be confirmed even by microspectro- scopic observation due to the very small volume (ca., 10 n\) obtained. O2 binding of the bloods Unlike other Hcs, A. castrensis He binds O2 non-cooperatively (, Fig. 2). The Hill coefficient («50), which is independent of pH, is (± SE, n == 11). Among the Hcs, A. castrensis also has an unprecedentedly high O2 affinity (Fig. 2), thus re- sembling tissue O2 carriers more than most blood O2 carriers. Unlike tissue O2 carri- ers, however, A. castrensis He has a small but significant normal Bohr shift. The slope of the regression line describing the data in Figure 2, or A log P5oM pH, is (± 95% )- HcO2 binding in A", proxima (which belongs to the same family as A. castrensis) is also non-cooperative ( ± SE; n =: 7) and it has a similar Bohr shift ( ± 95% ) although its O2 affinity is somewhat lower. The He of Y. limatula (which belongs to a different family) has a much lower O2 affinity, though still fairly high for a molluscan He, and it is moderately cooperative (Fig. 2). Its Bohr shift is indistinguishable from that of the other nuculoid Hcs ( ± ).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual r


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology