. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 376 FLOYD JOHN BRINLEY. curve represents the average of three to five tests. It may be seen from this figure that, with the exception of the two low concen- trations (M/225 and -M/3I3), the total amount of cyanide within the cell at equilibrium was the same for all the concentrations. The rate of entrance of cyanide increased with an increase in 70 60 ZSO LJ O LJ Q_ 30 20 I 0. ------ M\160 ----- M\225 - M\3I3 1,5 30 MINUTES 60 FIG. 8. Curve showing the effect of concentration of HCN upon the permeability of frog skin to cyan
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 376 FLOYD JOHN BRINLEY. curve represents the average of three to five tests. It may be seen from this figure that, with the exception of the two low concen- trations (M/225 and -M/3I3), the total amount of cyanide within the cell at equilibrium was the same for all the concentrations. The rate of entrance of cyanide increased with an increase in 70 60 ZSO LJ O LJ Q_ 30 20 I 0. ------ M\160 ----- M\225 - M\3I3 1,5 30 MINUTES 60 FIG. 8. Curve showing the effect of concentration of HCN upon the permeability of frog skin to cyanide. Concentrations of HCN used were M/iog, M/I24, M/i6o, M/i$6, M/22$, and M/3\3. Abscissae represents the time in minutes; ordinates per cent, cyanide. concentration. At a concentration of M/iog, equilibrium was reached within 30 minutes, while at lower concentrations equi- librium was not reached for a period of 45 minutes. The fact that the skin is not killed can be proved by substituting for the cyanide solution a mineral acid, which is known not to pass through living membranes, and testing the pH of the internal solu- tion. As there is no change in the intracellular acidity, it is evi- dent that no acid has passed through the skin. Although, it is known that the frog skin is not killed by the above treatment with HCN, it is desirable to ascertain what effect the cyanide does have upon it. A series of experiments was con- ducted to determine the effect of hydrogen cyanide on the po-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laborato
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
