. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHEMORECEPTION IN THE MUD SNAIL 97 LU > 90r 80 70 60 50 40 UJ 030 LU ^20 10 Water (o) f. C=M = Chloroform:methanol (2:1) C:M:W-Chloroform:methanol: water(3:48:47) MeOH-Methanol ETON = Ethanol 10 20 50 100 200 500 DOSE (/J/40ml test solution) 1000 UJ CO z o O_ CO LJ IT CD O or a_ FIGURE 3. Responses of N. obsoletus to shrimp components soluble in water and organic solvents. The experimental dosage-response values are indicated by symbols; numbers next to symbols indicate numbers of snails tested. Li
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHEMORECEPTION IN THE MUD SNAIL 97 LU > 90r 80 70 60 50 40 UJ 030 LU ^20 10 Water (o) f. C=M = Chloroform:methanol (2:1) C:M:W-Chloroform:methanol: water(3:48:47) MeOH-Methanol ETON = Ethanol 10 20 50 100 200 500 DOSE (/J/40ml test solution) 1000 UJ CO z o O_ CO LJ IT CD O or a_ FIGURE 3. Responses of N. obsoletus to shrimp components soluble in water and organic solvents. The experimental dosage-response values are indicated by symbols; numbers next to symbols indicate numbers of snails tested. Linear regression lines were drawn from com- puted effective dose values obtained by Potency Probit Analysis. The upright arrow over the regression line for water-soluble substances indicates that a 100% response was obtained with the largest dose tested. The inverted arrow beneath the regression line for methanol-soluble substances indicates that no response (0%) was obtained with the smallest dose tested. The potencies (and 95% confidence limits) of the indicated preparations were computed with respect to the potency of the preparation containing water soluble substances: C : M : W MeOH Upper lim. Potency Lower lim. Upper lim. Potency Lower lim. C:M ETOH Upper lim. Potency Lower lim. Upper lim. Potency Lower lim. extract. This measure insured that all groups of snails used in comparative tests were in the same physiological condition. Effect of heat treatment. The results of bioassays of heat-treated and untreated shrimp extract are given in Table T. Heat treatment had no detectable effect upon the stimulatory capacity of the extract. This conclusion was based upon a com parison of the response percentages in columns 2, 4, and 6 of the Table. Solubility studies. Figure 3 incorporates the experimental results of the solu- bility studies and the dosage-response regression lines obtained by a Potency Probit An
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
