. Animal tissue techniques. Histology -- Technique; Stains and staining (Microscopy); Histochemistry -- Technique. Hematoxylin Slainitig Procedures 133 bluing action. Then use Scott's soliuion (step II), a couple of drops of ammonia in a final wash, or a weak solution of sodium bicarbon- ate, to insure the alkaline condition. In the regressive method (be- low) the same applies, and as a precaution against neutralization caused by carrying one solution over into the other (step 5), after several exchanges of slides, add a drop or two of acid and base to them. If there is any pinkness left in th
. Animal tissue techniques. Histology -- Technique; Stains and staining (Microscopy); Histochemistry -- Technique. Hematoxylin Slainitig Procedures 133 bluing action. Then use Scott's soliuion (step II), a couple of drops of ammonia in a final wash, or a weak solution of sodium bicarbon- ate, to insure the alkaline condition. In the regressive method (be- low) the same applies, and as a precaution against neutralization caused by carrying one solution over into the other (step 5), after several exchanges of slides, add a drop or two of acid and base to them. If there is any pinkness left in the nuclei, they are not ade- quately blued. Regressive Method procedure: 1. Deparaffinize and run slides down (hydrate) to water, removing HgClo if present. 2. Stain in Delafield's hematoxylin, until slides are well overstained: 15-20 minutes. 3. Wash in running water: 3-5 minutes. 4. Transfer to 70% alcohol. 5. Destain in ^^ (app.) HCl (2-3 drops in ml. of 70% alcohol) few seconds. Remo\'e to 70%, alcohol with drop of ammonia added until sections turn blue. Examine under microscope. If nuclei are too dark, repeat above procedure. When nuclei stand out sharply blue against colorless background, blue slides thoroughly in alka- line solution: 3-5 minutes. 6. Transfer to 70% alcohol. 7. Counterstain. 8. Dehydrate as for progressive staining. 9. Clear and mount. results: nuclei—deep blue, sharper than in the progressive method other elements—colors of counterstain Mayer's Hematoxylin fixation: any general fixative or one specific for nuclear detail. solutions: Hematoxylin, page 125. Counterstains, page 129. procedure: 1. Deparaffinize and run slides down (hydrate) to water, removing HgCl. if Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Humason, Gretchen L. San Francisco, W. H. Freeman
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