. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 58 FREDERIK B. BANG number of observations were made on oysters in which a hole was carefully drilled near the pericardium and the shell was then picked away until the sheath was exposed. Observations on phagocytosis were made with a Zeiss phase microscope both at 500 X and 1250 X. A drop of freshly obtained blood was placed on a slide, then either a drop of bacterial suspensions from a freshly grown culture of marine bacteria was added to it, or a small portion of the colony itself was added with a loop directly to the drop
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 58 FREDERIK B. BANG number of observations were made on oysters in which a hole was carefully drilled near the pericardium and the shell was then picked away until the sheath was exposed. Observations on phagocytosis were made with a Zeiss phase microscope both at 500 X and 1250 X. A drop of freshly obtained blood was placed on a slide, then either a drop of bacterial suspensions from a freshly grown culture of marine bacteria was added to it, or a small portion of the colony itself was added with a loop directly to the drop of blood. The preparation was covered with a #1 cover-. anterior aorta- aortic bulb FIGURE 1. Diagram of half-shell preparation of oyster. Circulatory system indicated in heavy black line. Injections were made directly into the heart (H). Observations were made principally on mantle arteries. slip, and if observations were to be continued, the entire preparation was ringed with Vaseline to prevent evaporation. Amebocytes remained viable for 12 hours or more under these conditions. RESULTS Phagocytosis The amebocyte, which has been extensively studied in this and other molluscs (Faure-Fremiet, 1927), is a granular round cell floating freely in the blood stream. On contact with glass it flattens out and moves continually over the surface of the slide. This motion under phase may clearly be seen to begin by the extrusion of a. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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