. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 314 T. C. CHENG AND G. E. RODRICK. FIGURE 1. Graph showing effect of pH on the activity of the lysozyme in \vhole hemo- lymph of Mya arcnaria using M glycylglycine (black dots), M Tris-HCl (circles), M imidazole (squares), and M phosphate (triangles) as buffers. The lysozyme activity is expressed as AOD54o/min X 10~2 at 25° C. The effect of pH on the lytic activity of whole hemolymph is shown in Figure 1. When M glycylglycine, M imidazole, or M phosphate buffers are used, the optimal pH of the lys
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 314 T. C. CHENG AND G. E. RODRICK. FIGURE 1. Graph showing effect of pH on the activity of the lysozyme in \vhole hemo- lymph of Mya arcnaria using M glycylglycine (black dots), M Tris-HCl (circles), M imidazole (squares), and M phosphate (triangles) as buffers. The lysozyme activity is expressed as AOD54o/min X 10~2 at 25° C. The effect of pH on the lytic activity of whole hemolymph is shown in Figure 1. When M glycylglycine, M imidazole, or M phosphate buffers are used, the optimal pH of the lysozyme has been determined to be On the other hand, the optimal pH is when a M Tris-HCl buffer is employed. It is noted that a sharp peak is obtained at pH and relatively high lysozyme activities have beeen recorded between pH and when M glycylglycine and M imidazole are used. However, low activities and a relatively broad and nonspecific pH effect is observed when M Tris-HCl and M phosphate buffers are employed. As indicated in Table I, the major portion of the lysozyme activity is asso- ciated with the 4000 X g supernatant while only a small amount of activity is associated with the 4000 X g pellet. Similarly, when the 4000 X g supernatant is recentrifuged at 10,000 X g, most, if not all. of the lysozyme activity is associated with the supernatant. TABLE I Distribution of lysozyme activity in the hemolymph of Mya arenaria Sample AOD640 min Specific activity Whole hemolymph 4,000 X g supernatant 4,000 X g pellet 10,000 X g supernatant 10,000 X g pellet undctectable. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ).
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