. Pathological technique; a practical manual for workers in pathological histology and bacteriology. n solid media are at first pale gray or nearlywhite, and have a delicate fringe of radiating filaments attheir margins. Later, they spread widely over the surfaceof the media and form a layer or a membrane 1 or moremm. thick with wrinkled surface, which may become brownor black in color and of a velvety appearance. Down-growthmay occur into the underlying media. In stab cultures,lateral outgrowths occur along the line of inoculation. Gela-tine is slowly liquefied by the micro-organism. In bouil
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. n solid media are at first pale gray or nearlywhite, and have a delicate fringe of radiating filaments attheir margins. Later, they spread widely over the surfaceof the media and form a layer or a membrane 1 or moremm. thick with wrinkled surface, which may become brownor black in color and of a velvety appearance. Down-growthmay occur into the underlying media. In stab cultures,lateral outgrowths occur along the line of inoculation. Gela-tine is slowly liquefied by the micro-organism. In bouillon,growth appears as a downy sediment. The growth in the 374 PA THOL O GICAL TE CHNIQ UE. original cultures from the lesions may be slow, the coloniesappearing after a week or more. In succeeding generationsthe growth is more rapid. • ■4. * * . **»* . 0%* «*, & -** 1 *• • & -- * i. Fig. 114.—Sporotrichum Schenckii. Section of nodule in abdominal wall ofwhite rat. Grams stain. Cells and spores, the latter oblong and deeply colored ;X about 1000 (L. Hektoen and C. F. Perkins).. Fig. 115.—Sporotrichum Schenckii. Colonies on glycerin-agar plate. Lowpower (L. Hektoen and C. F. Perkins). Pathogenesis.—The micro-organism is pathogenic for ex-perimental animals, especially for white rats. In the latter PATHOGENIC BACTERIA AND FUNGI. 375 animals, septicemia, and disseminated suppurative or granu-lomatous lesions may be produced by inoculation with cul-tures. The testicles are very frequently involved after 1 Fig. 116.—Sporotrichum Schenckii. Margin of hanging-drop culture ; X about150 (L. Hektoen and C. F. Perkins).
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