. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LOCALIZATION OF LAMININ IN PLANARIAN 81. Ep; •<• Figures 2-4. Immunohistochemical localization oflaminin in the basal lamina. Reactive products are distributed along the basal lamina (arrowheads). Scale bar = 20 ^m. Figure 2. Intact. Figure 3. Intact: con- trol. Figure 4. Six-day regenerate. Ep. epidermal cell. In control sections, no reactive substances were seen in the basal lamina (Fig. 6). The stained material on the limiting layer appeared discontinuous. In the 6-day re- generate, when newly formed basal lamina appea
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LOCALIZATION OF LAMININ IN PLANARIAN 81. Ep; •<• Figures 2-4. Immunohistochemical localization oflaminin in the basal lamina. Reactive products are distributed along the basal lamina (arrowheads). Scale bar = 20 ^m. Figure 2. Intact. Figure 3. Intact: con- trol. Figure 4. Six-day regenerate. Ep. epidermal cell. In control sections, no reactive substances were seen in the basal lamina (Fig. 6). The stained material on the limiting layer appeared discontinuous. In the 6-day re- generate, when newly formed basal lamina appeared, the reactivity of the limiting layer was also evident, though the staining was slightly weak (data not shown). In both cases, there were no reactive products in the microfibrillar layer. The localization oflaminin in the basal lamina of intact and regenerating worms was compared by the PAG method. The labeling of laminin resulted in significant deposits in the limiting layers of both intact and regen- erated tissues (Figs. 7, 8). Fewer gold particles were in the microfibrillar layer than in the limiting layer. Discussion The planarian subepidermal basal lamina has varying degrees of development in different species (Bedini and Papi, 1974). The occurrence and constitution of the plan- arian basal lamina may not reflect the phylogenetic po- sition of animals, but rather unique functional significance of the basal lamina (Lindroos, 1991). Variations in thick- ness mainly relate to the extent to which the microfibrillar layer is developed (Sluys, 1989). For example, Rhabdo- coela have no microfibrils (Holt and Metrick, 1975), whereas some species of marine triclad have well-organ- ized microfibrillar layer (MacRae, 1965). The basal lam- ina of the former is similar to that of mammals, and the basal lamina of the latter is similar to that of amphibian larvae (Hay and Revel. 1963). In spite of such variations, the limiting layer can be seen in the basal laminae of most turbe
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
